Easy culturing of human ES/iPS cells in a biorelevant, defined, xeno-free environment

Recombinant human Laminin-521 is a protein matrix that has been shown to, for the first time, enable enzymatic passage of human ES and iPS cells as single cells with both sustained survival and long-term pluripotency

Until now, culturing human ES cells have been challenging to perform in a repeatable and defined fashion. Even more so, single-cell enzymatic passage of human ES cells without using artificial apoptosis inhibitors has been impossible. BioLamina now provides Laminin-521, a natural human ES cell niche matrix that solves this problem, allowing standardized ES cell culturing with almost no variability and without extensive training and costs of cell culture staff. In addition, by using the Laminin-521 ES cell culture method, automated high-throughput ES cell culturing can be done.Together with an appropriate cell culture medium, human recombinant laminin-511 offers several key advantages:


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Human ES and iPS cells can easily be passaged as single cells independent of rho kinase (ROCK) inhibitor treatment and survive at low plating densities. Survival of dissociated hES cells without ROCK inhibitor on human recombinant Laminin-521, mouse laminin-111 and Matrigel. Crystal Violet staining of hES cells 24 hours after single-cell suspension plating in mTeSR1 medium with and without Y-27632 treatment.	Human ES cell cultures, grown in a chemically defined, xeno-free environment on Laminin-521, passaged as single-cells in the absence of ROCK inhibitor expand continuously for months at an even better growth rate compared to cells grown on Matrigel passaged as clumps.	Pluripotent hES cells plated as single-cell suspension grow as a monolayer on top of Laminin-521. Laminin-521 mediates its effect via the α6β1 integrin, signaling through the PI3K/Akt pathway and the α6β1 integrin isoform is highly expressed in human ES cells. Laminin-521 has a very high affinity for α6β1 and, thus, Laminin-521 can convey the highest dose of biorelevant signal, resulting in increased single-cell survival. The long-term expanded cells homogenously express Oct4 (green) and remain karyotypically normal.

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Product	Description	Quantity / Price / Product No.	Add to cart

Laminin-521	Human recombinant protein, non-GMP

Easy culturing of human ES/iPS cells in a biorelevant, defined, xeno-free environment

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