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Laminin biology

read more about Biolaminas laminin cell culture substrates for culture of stem cells and primary cellsCELL-SPECIFIC CULTURE SUBSTRATES FOR IMPROVED CELL FUNCTIONALITY

BioLamina offer an expansive portfolio of recombinant laminin cell culture substrates, Biolaminins, for a variety of applications, including reliable expansion of human pluripotent stem cells (hESC and iPSC) and differentiation and maintenance of different specialized cell types, such as hepatocytes, skeletal muscle cells and different neural cells. All our matrices are chemically defined and animal component-free and allow you to imitate the natural cell-matrix interactions in vitro. The authentic cell culture environment provides more consistent and reliable cellular responses and improves cell functionality. The impact of our laminin matrices on the advances in cell based research and cell therapy have been published in many high-impact journals. 

                         
READ MORE ABOUT THE DIFFERENT BIOLAMININ CELL CULTURE APPLICATIONS

 


LAMININS ARE KEY PROTEINS IN THE BASEMENT MEMBRANE

Basement membranes (BM) are sheet-like extracellular matrix structures that is the foundation for cells to grow on. The BM composition is highly cell-surface selective and laminins are the key proteins, surrounding virtually all cells. Laminins are important for embryogenesis and in the adult body, the expression is tissue-specific.


There are many binding sites on the full-length laminin molecule which can interact with cell membrane receptors. 
Integrins are important laminin receptors and the laminin-integrin interaction triggers vital intracellular signaling pathways. Via binding to specific cellular receptors, laminins regulate cellular responses, such as cell anchorage, survival, proliferation, migration, organization and specialization, leading to improved cell functionality. Without
the right combination of laminin isoforms cells and tissues becomes dysfunctional. Laminins are also capable of co-signaling with growth factors and efficiently buffers endogenously produced growth factors, which adds to the mechanistic complexity. 

 


ONLY THE INTACT, FULL LENGTH LAMININ MOLECULES CAN REPLICATE THE BIOLOGICAL RELEVANT CONDITIONS

Laminin molecular structure cell cultureLaminins are multidomain, heterotrimeric, glycoproteins, composed of three different subunits; an α-chain, β-chain and γ-chain. The assembled laminin molecule forms a cross-like structure. There are to date at least 16 distinct isoforms identified in mammals and their names derive from their combination of the subunits, thus, laminin isoform 521 has chain composition alpha-5/beta-2/gamma-1. It has been suggested that the molecular mechanism underlying the laminin-mediated signaling involves the C-terminal region of laminins but integrins and other cellular receptors also interact with some of the N-terminal globular domains.

BioLamina's Biolaminin substrates are the only original full length, recombinant laminins on the market. A fractionated or truncated laminin molecule or laminins isolated from tissue, lack many of the laminin domains which is needed by the cells for the proper extracellular network to form and to for stimulation of correct cellular signal transductions. Hence, only the intact, full length laminin can create a more authentic cell culture environment.


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More articles HERE

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Biolaminin 521 MX research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.