PRODUCT OVERVIEW

Biolaminin 121 LN

Biolaminin 121 LN (LN121)

Human recombinant laminin 121


Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue-specific. Laminins 111 and 121 are widely expressed during embryogenesis, important for early epithelial development and differentiation of the epiblast thus vital for the development of an embryo. However, the distribution after birth is restricted to only a few tissues, such as the brain and kidney. 

Biolaminin 121 LN (LN121) can be used as a general attachment protein for most cell types in vitro and in particular for hepatic and neural differentiation, and to enhance neurite outgrowth. 

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100 µg 1 x 1 ml €78
500 µg 1 x 5 ml €339
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SIZE GUIDE

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Applications Supporting data Instructions Technical info

Laminin-121 is one of the α1 chains containing laminins and the other isoform is laminin-111. In the adult, laminin α1 chain expression is relatively restricted and is only present in some epithelial basement membranes, and primarily found in tissues such as the eye, liver, and kidney, whereas hardly expressed at all in endothelial, adipose, nervous, and muscle tissues (Ekblom, 2003; Virtanen, 2000).

Biolaminin 121 LN (LN121) can support the survival, proliferation, and differentiation of many different cell types in vitro. Biolaminin 121 LN (LN121) attachment and proliferation of for most cell types in vitro and are particularly useful for hepatic and neural differentiation. Biolaminin 121 also effectively supported neurite outgrowth. Biolaminin 121 has a higher affinity to integrin receptors compared to Biolaminin 111 with the highest affinity to α6β1 and α7β1 integrins (Sasaki, 2010).

Commonly, laminin 111 has been used as a general attachment protein for most cell types in vitro. Laminin 121-based cell culture substrates have not been commercially available until now and therefore the functional comparison between LN121, LN111 and other laminins isoforms is yet to be performed in order to understand the potential for LN121 for cells in culture.

However, known is that the laminin beta 2 chain has been suggested to have a higher affinity for integrins compared to the alfa 1 chain thus influencing the stability of integrin-laminin and activation of downstream effectors in cell signaling cascades.

 

It is unknown why laminin 121 can exert all these major biological effects on such a wide selection of cell types, but one explanation is that, depending on the cell type, laminin 121 can not only induce different cell signaling cascades but as all laminins also co-signal with various growth factors.

However, by coating your plate with recombinant Biolaminin 121 you support your cells with a defined and animal origin-free substrate. Therefore, LN121 enables better control of your cell-matrix and experiments.

 

Laminin121 – Recombinant expression and interactions with integrins
Sasaki T., Takagi J., Giudici C., Yamada Y., Arikawa-Hirasawa E., Deutzmann R., Timpl R., Sonnenberg A., Bächinger H.P., Tongeh D.
Matrix Biol., 2010

In this article, the authors compared laminin-121 to laminin-111. Laminin-121 showed the highest affinity to α6β1 and α7β1 integrins and furthermore, laminin-121 most effectively supported neurite outgrowth. This suggests that the β2 laminins have a higher affinity for integrins than the β1 laminins.

COATING PLATES

See instructions for coating protocol, recommended coating volumes, and concentrations.

COATING PROCEDURE

1. Slowly thaw recombinant laminins at +4°C before use. 

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes, and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spread evenly across the surface. Note that the laminin matrix will be inactivated if let dry.

IMPORTANT NOTES

  • The laminin stock solution is long-term stable when stored at -20°C to -80°C. Please refer to the product-specific CoA for shelf life details.

  • Repeated freeze-thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. Thawed, undiluted laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions.

  • Avoid long exposure of the protein to ambient temperatures.

  • For your convenience, the coated plates can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

  • The protocol can easily be made totally defined and animal origin-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

Product name

Biolaminin 121 LN

 


Product code

LN121

 


Declaration

For research use only

 


Documents

SDS

CoA

Storage

-20°C to -80°C

 


Stock concentration

0.1mg/ml

 


Appearance

Clear, colorless, buffered solution with a 
pH of 7.2 with 10% glycerol and 0.02% NaN3

 

Shipping Condition

Dry Ice

Protein name

Laminin 121 (Laminin-3)

 


Classification

Defined and animal origin-free, human 
recombinant protein 

 

Product application

General cell culture substrate. Can be found in the placenta, skeletal muscles, and kidneys.