Science Room

PRODUCT RECOMMENDATION

Endothelial cells: 
LAMININ-511
Pericytes: 
LAMININ-111 or LAMININ-211
Astrocytes: 
LAMININ-111LAMININ-211 or
  LAMININ-521


                                             Go to the BioLamina shop

Do you want us to contact you? Please klick here


REFERENCES

HOW TO CULTURE BLOOD-BRAIN BARRIER CELLS ON LAMININ SUBSTRATES

Neural stem cell culture on laminin-521Blood-brain barrier structure and function

Endothelia cells lining small brain capillaries separate the blood from the brain parenchyma. They function like a barrier, the blood brain barrier (BBB), due to tight junctions and different solute carriers and efflux transporters.

The endothelial cells are covered by pericytes embedded in basement membrane, and pericytes and endothelium are in turn completely covered by a layer of astrocyte end feet. The brain capillary endothelial cells, pericytes, astrocytes and associated basement membrane is called the neurovascular unit.

There is a great interest for the development of an ideal in vitro model reflecting authentic BBB properties and function, allowing both mechanistic studies and rapid screening of permeable CNS drug candidates.

 

Laminins for each cell type relevant to the neurovascular unit

  • Endothelial cells express laminin-411 and laminin-511 (Hallman, 2005)
  • Pericyte differentiation is regulated by laminin-111 and laminin-211 (Yao, 2014)
  • Astrocytes express laminin-111, laminin-211 and laminin-521 (Sixt, 2001)

 

Laminins relevant for BBB integrity

Strickland group (Yao., 2014) demonstrate that laminin-111 and laminin-211 are essential for the maintenance of the BBB integrity by regulating differentiation of pericytes. In support, Menezes and colleagues (2014) report that mice lacking alfa2 have a defective BBB. In another article, the authors show that laminin-511 plays a key role in maintenance and repair of BBB after hypoxic injury and inflammation (Kangwantas, 2016).

 

We recommend LN511/521, LN111 and LN211 for maintenance of BBB cells in culture.

LAMININ KEY ADVANTAGES

  •  Endothelial cells express laminin-411 and laminin-511

  • Pericyte differentiation is regulated by laminin-111 and laminin-211

  • Astrocytes express laminin-111, laminin-211 and laminin-521 

  • Laminin-111 and laminin-211 are essential for the maintenance of the BBB integrity

  • Laminin-511 plays a key role in maintenance and repair of BBB after hypoxic injury and inflammation
  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.