Science Room

PRODUCT RECOMMENDATION

Endothelial cells: 
BIOLAMININ 511
Pericytes: 
BIOLAMININ 111 or BIOLAMININ 211
Astrocytes: 
BIOLAMININ 111BIOLAMININ 211 or
  BIOLAMININ 521


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REFERENCES

HOW TO CULTURE BLOOD-BRAIN BARRIER CELLS ON BIOLAMININ SUBSTRATES

Neural stem cell culture on laminin-521Blood-brain barrier structure and function

Endothelia cells lining small brain capillaries separate the blood from the brain parenchyma. They function like a barrier, the blood brain barrier (BBB), due to tight junctions and different solute carriers and efflux transporters.

The endothelial cells are covered by pericytes embedded in basement membrane, and pericytes and endothelium are in turn completely covered by a layer of astrocyte end feet. The brain capillary endothelial cells, pericytes, astrocytes and associated basement membrane is called the neurovascular unit.

There is a great interest for the development of an ideal in vitro model reflecting authentic BBB properties and function, allowing both mechanistic studies and rapid screening of permeable CNS drug candidates.

 

Biolaminin for each cell type relevant to the neurovascular unit

  • Endothelial cells express laminin 411 and laminin 511 (Hallman, 2005)
  • Pericyte differentiation is regulated by laminin 111 and laminin 211 (Yao, 2014)
  • Astrocytes express laminin isoforms 111, 211 and 521 (Sixt, 2001)

 

Laminins relevant for BBB integrity

Strickland group (Yao., 2014) demonstrate that laminin 111 and laminin 211 are essential for the maintenance of the BBB integrity by regulating differentiation of pericytes. In support, Menezes and colleagues (2014) report that mice lacking alfa2 have a defective BBB. In another article, the authors show that laminin 511 plays a key role in maintenance and repair of BBB after hypoxic injury and inflammation (Kangwantas, 2016).

 

BIOLAMININ KEY ADVANTAGES

  • Endothelial cells express laminin isoforms 411 and 511

  • Pericyte differentiation is regulated by laminin 111 and laminin 211

  • Astrocytes express laminin isoforms 111, 211 and 521 

  • Laminin 111 and laminin 211 are essential for the maintenance of the BBB integrity

  • Laminin 511 plays a key role in maintenance and repair of BBB after hypoxic injury and inflammation
  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG human stem cell matrix makes pluripotent stem cell culture easy in a defined, animal component-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Laminin 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN (LN521) human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.