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Product recommendation

LN521

Corneal endothelial cells (CECs) are highly polarized flat cells that form a uniform monolayer of the innermost layer of the cornea, separating the cornea from the aqueous humor that fills the anterior chamber. CECs plays several essential roles in corneal homeostasis and are specialized in regulating corneal hydration and transparency.

 

Laminin are efficient coating substances that improve the yield of in vitro CEC cultures

The basal surface of the CSCs rest on an amorphous collagenous membrane, Descemet’s membrane, composed of type IV collagen, type VIII collagen, perlecan, nidogens, laminin-332, laminin-411, and laminin-511 (Kabosova et al., 2007). Integrin α3β1 is the only protein found exclusively at the basal surface of the CSCs, forming an almost homogenous layer (He et al., 2016) and ligands of integrin α3β1, such as laminin-332, laminin-511, and laminin-521 are efficient coating substances that improve the yield of in vitro CEC cultures.

 

Laminin-521 and -511 for the isolation and expansion of corneal endothelial progenitors

Corneal endothelial progenitors are efficiently isolated and expanded on laminin-521 and laminin-511 (Hara et al., 2014). Hara and colleagues demonstrate that the proliferative capacity of these endothelial progenitors is very high on laminin-511 compared to conventional methods. Laminin-511 can thus be used to rapidly isolate and expand a homogenous population of endothelial progenitors that can be differentiated to endothelial cells in a serum free environment.

Recommended publications

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.