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LN521

Corneal endothelial cells (CECs) are highly polarized flat cells that form a uniform monolayer of the innermost layer of the cornea, separating the cornea from the aqueous humor that fills the anterior chamber. CECs plays several essential roles in corneal homeostasis and are specialized in regulating corneal hydration and transparency.

 

Laminin are efficient coating substances that improve the yield of in vitro CEC cultures

The basal surface of the CSCs rest on an amorphous collagenous membrane, Descemet’s membrane, composed of type IV collagen, type VIII collagen, perlecan, nidogens, laminin-332, laminin-411, and laminin-511 (Kabosova et al., 2007). Integrin α3β1 is the only protein found exclusively at the basal surface of the CSCs, forming an almost homogenous layer (He et al., 2016) and ligands of integrin α3β1, such as laminin-332, laminin-511, and laminin-521 are efficient coating substances that improve the yield of in vitro CEC cultures.

 

Laminin-521 and -511 for the isolation and expansion of corneal endothelial progenitors

Corneal endothelial progenitors are efficiently isolated and expanded on laminin-521 and laminin-511 (Hara et al., 2014). Hara and colleagues demonstrate that the proliferative capacity of these endothelial progenitors is very high on laminin-511 compared to conventional methods. Laminin-511 can thus be used to rapidly isolate and expand a homogenous population of endothelial progenitors that can be differentiated to endothelial cells in a serum free environment.

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Biolaminin 521 CTG

Biolaminin 521 CTG human stem cell matrix makes pluripotent stem cell culture easy in a defined, animal component-free and biologically relevant cell culture system.

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Biolaminin 521 MX

Laminin 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN (LN521) human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.