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BIOLAMININ 521, BIOLAMININ 211 and BIOLAMININ 111 

 
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How to culture cortical neurons on Biolaminin substrates

hPSC-derived clinically compliant dopaminergic neuron differentiation and culture on defined human recombinant laminin-521 and laminin-111 cell culture reagentsLaminins are important for cortical neuron development

Laminins are key extracellular matrix proteins, expressed primarily in the basement membrane associated with the pia mater of the cerebral cortex and in the ventricular zone, subplate and marginal zone of the developing cerebral wall. Several different laminin isoforms that contain the β2 and γ3 chains, such as laminin 521, has been isolated from the brain, underlining their indispensable role in CNS function (Radner, 2012). For example, β2 and γ3 chain laminins are pivotal for a functional cortical pial basement membrane to which Cajal Retzius and radial glial cells attach. Defects in their expression have been linked to cortical laminar disorganization (Radner, 2012). In addition, knockout of laminin γ1 in the cerebral cortex leads to defects in neuritogenesis and neuronal migration (Chen, 2009).

 


Laminin-integrin interaction is crucial for development, function, organization, and polarization

Interaction of laminins with β1 integrin receptors is crucial not only for axon initiation and development but also for the cytoskeleton remodeling occurring during neuronal polarization (Lei, 2012; Nasu, 2012). Cortical abnormalities have been linked to transgenic α6β1 and α3β1 receptors (Colognato, 2005), both key receptors of laminin 111, 211, 511 and 521.


Biolaminin substrates support the long-term culture of cortical neurons

Primary cortical neurons cultured on lamininApart from controlling neurite development, laminins also guide axons of single multipolar cortical neurons in vitro and serve as a good model for mimicking the in vivo protein gradient conditions involved in creating defined neural networks during CNS development (Fricke, 2011). Human recombinant Biolaminin 521 and Biolaminin 211 substrates from BioLamina has been shown to provide excellent support for the long-term culture (>80 days) of human primary cortical neurons (unpublished data). In addition to laminin 521 and laminin 211, laminin 111 also allows both firm attachment and extensive neurite outgrowth and induction of Synapsin I expression in primary cortical neurons (Savettieri, 1998). 

Human primary cortical neurons cultured on hr laminin.
Courtesy of Julia Abele, Leibniz Institute for Neurobiology.


Biolaminin support differentiation of human PSC into neocortical neural stem cells and cortical neurons

Biolaminin substrates provide excellent support for the differentiation of human pluripotent stem cells (PSCs) into cerebral neocortical neural stem cells (NSCs) and functional cortical neurons (Shi, 2012). Laminin substrates also play an important role in the development of a human induced pluripotent stem cells (hiPSCs) and astrocytes co-culture model, recapitulating sustained activity, bursting frequency and calcium oscillation synchronization of cortical neurons over time (Kuijlaars, 2016).

In a recent publication in Scientific Reports by Hyvärinen (2019), the authors report that the differentiation of hPSC-derived cortical cultures on defined human recombinant Biolaminin 521 (LN521) substrates is repeatable and results in the generation of cortical neurons, which form functionally active networks. The hPSC-derived networks developed synchronous activity, which involved glutamatergic and GABAergic inputs, recapitulating the classical cortical activity also observed in rodent counterparts. Principal component analysis (PCA) based on spike rates, network synchronization, and burst features revealed the segregation of hPSC-derived and rat network recordings into different clusters, reflecting the species-specific and maturation state differences between the two networks. Overall, hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, which validates their applicability as a human-specific model for pharmacological studies and modeling network dysfunctions.  

KEY BENEFITS WITH BIOLAMININ SUBSTRATES FOR CULTURE OF CORTICAL NEURONS

  • A key component of the basal lamina in the pia mater and in the ventricular zone

  • Play an important role in neocortex development, directional axon initiation, and development of cortical neurons

  • Promote neural polarization via interaction with integrin β1 mediated microtubule assembly and stabilization

  • Cortical abnormalities have been linked to defects in the α6β1 and α3β1 integrin, key receptors for laminin isoforms 111, 211, 511 and 521

  • Biolaminin 521 and 211 support long-term culture of primary human cortical neurons

  • Laminin isoforms 521, 211 and 111 plays a major role in neurite initiation, growth and subsequent maturation of cortical neurons in vitro

  • Biolaminin 111 promotes formation and stabilization of continuous cortical neuroepithelium from mESCs

  • A defined and animal origin-free cell culture matrix for clinical compliance
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  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal origin-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Biolaminin 521 MX research-grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal origin-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN research-grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal origin-free and biologically relevant cell culture system for better cell models.