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PRODUCT RECOMMENDATION

LAMININ-521, LAMININ-211 and LAMININ-111 

 
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CUSTOMER TESTIMONIALS          

How to culture cortical neurons on laminin substrates

hPSC-derived clinically compliant dopaminergic neuron differentiation and culture on defined human recombinant laminin-521 and laminin-111 cell culture reagentsLaminins are important for cortical neuron development

Laminins are key extracellular matrix proteins, expressed primarily in the basement membrane associated with the pia matter of the cerebral cortex and in the ventricular zone, subplate and marginal zone of the developing cerebral wall. Several different laminin isoforms that contains the β2 and γ3 chains, such as laminin-521, has been isolated from the brain, underlining their indispensable role in CNS function (Radner, 2012). For example, β2 and γ3 chain laminins are pivotal for a functional cortical pial basement membrane to which Cajal Retzius and radial glial cells attach. Defects in their expression has been linked to cortical laminar disorganization (Radner, 2012). In addition, knockout of laminin γ1 in the cerebral cortex leads to defects in neuritogenesis and neuronal migration (Chen, 2009).


Laminins-integrin interaction are crucial for development, function, organization and polarization

Interaction of laminins with β1 integrin receptors are crucial not only for axon initiation and development, but also for the cytoskeleton remodelling occurring during neuronal polarization (Lei, 2012; Nasu, 2012). Cortical abnormalities have been linked to transgenic α6β1 and α3β1 receptors (Colognato, 2005), both key receptors of laminin-111, -211, -511 and -521.


Laminin substrates support long-term culture of cortical neurons

Primary cortical neurons cultured on lamininApart from controlling neurite development, laminins also guide axons of single multipolar cortical neurons in vitro and serves as a good model for mimicking the in vivo protein gradient conditions involved in creating defined neural networks during CNS development (Fricke, 2011). Laminin-521 and laminin-211 from BioLamina has been shown to provide excellent support for the long-term culture (>80 days) of human primary cortical neurons (unpublished data). In addition to laminin-521 and laminin-211, laminin-111 also allows both firm attachment and extensive neurite outgrowth and induction of Synapsin I expression in primary cortical neurons (Savettieri, 1998). 

Human primary cortical neurons cultured on hr laminin.
Courtesy of Julia Abele, Leibniz Institute for Neurobiology.


Laminin support differentiation of human PSC into neocortical neural stem cells and cortical neurons

Laminin coated substrates provides excellent support for differentiation of human pluripotent stem cells (PSCs) into cerebral neocortical neural stem cells (NSCs) and functional cortical neurons (Shi, 2012). Laminin also play an important role in the development of a human induced pluripotent stem cells (hiPSCs) and astrocytes co-culture model, recapitulating sustained activity, bursting frequency and calcium oscillation synchronization of cortical neurons over time (Kuijlaars, 2016).

LAMININ ADVANTAGES

  • Key component of the basal lamina in the pia mater and in the ventricular zone

  • Play an important role in neocortex development, directional axon initiation and development of cortical neurons

  • Promote neural polarization via interaction with integrin β1 mediated microtubule assembly and stabilization

  • Cortical abnormalities have been linked to defects in the α6β1 and α3β1 integrin, key receptors for laminin-111, -211, -511 and -521

  • Laminin-521 and -211 support long-term culture of primary human cortical neurons

  • Laminin-521, -211 and -111 plays a major role in neurite initiation, growth and subsequent maturation of cortical neurons in vitro

  • Laminin-111 promotes formation and stabilization of continuous cortical neuroepithelium from mESCs

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  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

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  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

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  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.