Science Room

Show Menu

PRODUCT RECOMMENDATION

BIOLAMININ 521, BIOLAMININ 211 and BIOLAMININ 111 

 
                                             Go to the BioLamina shop
 

Do you want us to contact you? Please klick here


CUSTOMER TESTIMONIALS          

How to culture cortical neurons on Biolaminin substrates

hPSC-derived clinically compliant dopaminergic neuron differentiation and culture on defined human recombinant laminin-521 and laminin-111 cell culture reagentsLaminins are important for cortical neuron development

Laminins are key extracellular matrix proteins, expressed primarily in the basement membrane associated with the pia matter of the cerebral cortex and in the ventricular zone, subplate and marginal zone of the developing cerebral wall. Several different laminin isoforms that contains the β2 and γ3 chains, such as laminin 521, has been isolated from the brain, underlining their indispensable role in CNS function (Radner, 2012). For example, β2 and γ3 chain laminins are pivotal for a functional cortical pial basement membrane to which Cajal Retzius and radial glial cells attach. Defects in their expression has been linked to cortical laminar disorganization (Radner, 2012). In addition, knockout of laminin γ1 in the cerebral cortex leads to defects in neuritogenesis and neuronal migration (Chen, 2009).


Laminin-integrin interaction are crucial for development, function, organization and polarization

Interaction of laminins with β1 integrin receptors are crucial not only for axon initiation and development, but also for the cytoskeleton remodelling occurring during neuronal polarization (Lei, 2012; Nasu, 2012). Cortical abnormalities have been linked to transgenic α6β1 and α3β1 receptors (Colognato, 2005), both key receptors of laminin 111, 211, 511 and 521.


Biolaminin substrates support long-term culture of cortical neurons

Primary cortical neurons cultured on lamininApart from controlling neurite development, laminins also guide axons of single multipolar cortical neurons in vitro and serves as a good model for mimicking the in vivo protein gradient conditions involved in creating defined neural networks during CNS development (Fricke, 2011). Human recombinant Biolaminin 521 and Biolaminin 211 substrates from BioLamina has been shown to provide excellent support for the long-term culture (>80 days) of human primary cortical neurons (unpublished data). In addition to laminin 521 and laminin 211, laminin 111 also allows both firm attachment and extensive neurite outgrowth and induction of Synapsin I expression in primary cortical neurons (Savettieri, 1998). 

Human primary cortical neurons cultured on hr laminin.
Courtesy of Julia Abele, Leibniz Institute for Neurobiology.


Biolaminin support differentiation of human PSC into neocortical neural stem cells and cortical neurons

Biolaminin coated substrates provides excellent support for differentiation of human pluripotent stem cells (PSCs) into cerebral neocortical neural stem cells (NSCs) and functional cortical neurons (Shi, 2012). Laminin substrates also play an important role in the development of a human induced pluripotent stem cells (hiPSCs) and astrocytes co-culture model, recapitulating sustained activity, bursting frequency and calcium oscillation synchronization of cortical neurons over time (Kuijlaars, 2016).

BIOLAMININ ADVANTAGES

  • Key component of the basal lamina in the pia mater and in the ventricular zone

  • Play an important role in neocortex development, directional axon initiation and development of cortical neurons

  • Promote neural polarization via interaction with integrin β1 mediated microtubule assembly and stabilization

  • Cortical abnormalities have been linked to defects in the α6β1 and α3β1 integrin, key receptors for laminin isoforms 111, 211, 511 and 521

  • Biolaminin 521 and 211 support long-term culture of primary human cortical neurons

  • Laminin isoforms 521, 211 and 111 plays a major role in neurite initiation, growth and subsequent maturation of cortical neurons in vitro

  • Biolaminin 111 promotes formation and stabilization of continuous cortical neuroepithelium from mESCs

  • A defined and animal component-free cell culture matrix for clinical compliance
  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Biolaminin 521 MX research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.