1. When thawing ESC cells grown on the laminin what is the recovery like?

The post-thaw cell survival is high when supported by LN521™, especially when freeze-thawed as single cells. This is nicely described in a recent publications by Miyazaki and colleagues where they show that hPSC that were freeze-thawed a s colonies showed markedly decreased survival compared to cells freeze-thawed as single cells which retained the majority of their viability (Miyazaki et al., 2013).