Science Room

PRODUCT RECOMMENDATION

Oligodendrocytes: LAMININ-211
Schwann cells: LAMININ-211LAMININ-411
Astrocytes: LAMININ-111, LAMININ-211LAMININ-521


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REFERENCES

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CUSTOMER TESTIMONIALS          

 

HOW TO CULTURE GLIAL CELLS ON LAMININ SUBSTRATES

Neural stem cell culture on laminin-521Astrocytes

Astrocytes express laminin-111, laminin-211 and laminin-521 (Sixt, 2001; Jucker, 1996). Yao and colleagues (Yao, 2014) showed that laminin-111 and laminin-211 are essential for the maintenance of the blood brain barrier integrity by regulating differentiation of pericytes. In another recent publication, it was shown that laminin-521 is indispensable for retinal angiogenesis and astrocyte migration (Gnanaguru, 2013).

 

Oligodendrocytes

Relucio and colleagues (Relucio, 2009) showed that α2-laminins (laminin-211 and laminin-221) are required for the maturation of oligodendrocytes. Moreover, and in support, lama2-mutant mice exhibit CNS myelination defects (congenital muscular dystrophy) due to impaired oligodendrocyte maturation (Chun, 2003).

 

Schwann cells

Schwann cells, responsible for the formation of myelin sheaths around nerve axons in the peripheral nervous system, require laminin-411 and laminin-211 to spread and sort axons (Pellegatta, 2013). In addition, Petersen and colleagues (Petersen, 2015) demonstrate and provide evidence of the fundamental role of laminin-211 in Schwann cell biology as a regulator of radial sorting, driving the terminal differentiation and thus, myelination of axons.

Further adding to this evidence is a mechanistic article just published by Poitelon et al. (2016) in Nature Neuroscience, in which the authors show that laminin-211 in combination with mechanical stimuli activate Yap and Taz, downstream effectors in the Hippo pathway, required for radial sorting and myelination. Apart from promotion, laminin-211 can also inhibit myelination by modulating neuregulin 1 type III activity via PKA signaling and thereby prevent inappropriate and excessive myelin wrapping of small nerve fibers (Ghidinelli, 2017). 

LAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on laminin-521.

  • Astrocytes express laminin-111, laminin-211 and laminin-521

  • Laminin-211 and laminin-221) are required for the maturation of oligodendrocytes

  • Laminin-211 and laminin-411 have a fundamental role in Schwann cell biology

  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment for human ES and iPS cell expansion and differentiation

  • No lot-to-lot variability for standardized experiments with less variation

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.