Science Room

PRODUCT RECOMMENDATION

Oligodendrocytes: BIOLAMININ 211
Schwann cells: BIOLAMININ 211BIOLAMININ 411
Astrocytes: BIOLAMININ 111, BIOLAMININ 211BIOLAMININ 521


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HOW TO CULTURE GLIAL CELLS ON BIOLAMININ SUBSTRATES

Neural stem cell culture on laminin-521Astrocytes

Astrocytes express laminin isoforms 111, 211 and 521 (Sixt, 2001; Jucker, 1996). Yao and colleagues (Yao, 2014) showed that laminin 111 and laminin 211 are essential for the maintenance of the blood brain barrier integrity by regulating differentiation of pericytes. In another recent publication, it was shown that laminin 521 is indispensable for retinal angiogenesis and astrocyte migration (Gnanaguru, 2013).

hiPSC-derived astrocytes provide a promising source of astrocytes for screening models, but generally the protocols are extensive and use undefined conditions, introducing variability. A recent publication by Lundin et al., the authors report a protocol for human induced pluripotent stem cell (hiPSC)-derived astroglia (NES-Astro) developed under defined conditions through long-term neuroepithelial-like stem (ltNES) cells (Lundin, 2018). The authors show high reproducibility across hiPSC lines, and proven astrocyte functions including SLC1A3-driven glutamate uptake, immune competence, and calcium responsiveness to neurotransmitters. With the use of human recombinant Biolaminin 521, they also show the use of an animal-free culture system applicable for good manufacturing practice and clinical adaptation. 

 

Oligodendrocytes

Relucio and colleagues (Relucio, 2009) showed that α2-laminins (laminin 211 and laminin 221) are required for the maturation of oligodendrocytes. Moreover, and in support, lama2-mutant mice exhibit CNS myelination defects (congenital muscular dystrophy) due to impaired oligodendrocyte maturation (Chun, 2003).

 

Schwann cells

Schwann cells, responsible for the formation of myelin sheaths around nerve axons in the peripheral nervous system, require laminin isoforms 411 and 211 to spread and sort axons (Pellegatta, 2013). In addition, Petersen and colleagues (Petersen, 2015) demonstrate and provide evidence of the fundamental role of laminin 211 in Schwann cell biology as a regulator of radial sorting, driving the terminal differentiation and thus, myelination of axons.

Further adding to this evidence is a mechanistic article just published by Poitelon et al. (2016) in Nature Neuroscience, in which the authors show that laminin 211 in combination with mechanical stimuli activate Yap and Taz, downstream effectors in the Hippo pathway, required for radial sorting and myelination. Apart from promotion, laminin 211 can also inhibit myelination by modulating neuregulin 1 type III activity via PKA signaling and thereby prevent inappropriate and excessive myelin wrapping of small nerve fibers (Ghidinelli, 2017). 

BIOLAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on Biolaminin 521.

  • Astrocytes express laminin isoforms 111, 211 and 521

  • Laminin 211 and laminin 221 are required for the maturation of oligodendrocytes

  • Laminin 211 and laminin 411 have a fundamental role in Schwann cell biology

  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment for human ES and iPS cell expansion and differentiation

  • No lot-to-lot variability for standardized experiments with less variation

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG human stem cell matrix makes pluripotent stem cell culture easy in a defined, animal component-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Laminin 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN (LN521) human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.