hESC derived Hepatocytes
Recent study takes hES derived liver cell a big step closer to therapy: Scientists at the University of Edinburgh present results for ES derived hepatocyte differentiation on human recombinant laminins that represent significant improvement of metabolic activity and functional organisation:
Cameron K., Tan R., Schmidt-Heck W., Campos G., Lyall M.J, Wang Y., Lucendo-Villarin B., Szkolnicka D., Bates N., Kimber S.J., Hengstler J.G., Godoy P., Forbes S.J., Hay D.C.
Stem Cell Reports, 2015
Human ES cells cultured on human recombinant laminin-521 and laminin-111 show efficient hepatocyte specification, maturation, function and stabilization of phenotype. The results presented in the paper represents a significant advance compared to any previous published data especially regarding metabolic activity and functional organization. Cells cultured on the laminin matrices exhibited significantly increased metabolic function relative to cells on matrigel and human primary hepatocytes. The laminin cultured hepatocyte-like cells were arranged in lobule like structures, reminiscent of regenerating liver, with positive staining for MRP1 and MRP2 and were capable of biliary efflux.
See Dr Kate Cameron's presentation
The increasing need of livers for transplantation combined with the ongoing shortage has focused attention to cell based therapies to support and regenerate liver function. Pluripotent stem cells provide an alternate, unlimited source of somatic cells for clinical application.
Our work focuses on deriving hepatic progenitors and hepatocytes from embryonic stem (ES) cells. These can be generated under GMP conditions and using a serum free, defined procedure, differentiated to hepatic progenitor cells, and to functional hepatocytes. Recently this protocol has been translated onto laminins, making the whole process GMP translatable. Furthermore, differentiation on laminin-521 and laminin-111 produces more mature, functional hepatcoytes.
Find out and keep updated on how to use laminins for hepatic applications