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LAMININ-521


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REFERENCES

HOW TO CULTURE HIPPOCAMPAL NEURONS ON LAMININ SUBSTRATES

Neural stem cell culture on laminin-521

Laminin expression in the hippocampus

The hippocampal basement membrane mainly contains alpha-5 laminins (laminin-521 and laminin-511) and has been shown to protect neurons from apopotosis (Chen et al., Mol Biol Cell, 2003; Indyk et al., Neuroscience, 2003).

 

Laminin for hippocampal cell culture

A recent study showed that laminin-511 promoted elongation of both axons and dendrites of hippocampal neurons in vitro (Fusaoka-Nishioka etal., Neurosci Res., 2011). The laminin isoform -521 only recently became available and hippocampal cells have since then been grown successfully on laminin-521 (unpublished results). As both laminin-521 and laminin-511 are expressed in most adult stem cell niches they likely also support neural progenitors in the dentate gyrus.

LAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on laminin-521.

  • Hippocampal basement membrane mainly contains laminin-521 and laminin-511

  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment 

  • No lot-to-lot variability for standardized experiments with less variation

  • Effective proliferation of human neural stem cells

  • Laminin-521 triggers the authentic cell signaling pathways which generates hPSCs with a more uniform gene expression profile

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.