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HSC & blood cell applications

PRODUCT RECOMMENDATION

LAMININ-521


Buy human recombinant laminin-521 cell culture reagents at BioLamina                                             Go to the BioLamina shop

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PROTOCOL

Generation of iPSC-derived megakaryocytes and platelets


Red blood cell generation from human pluripotent stem cells
  


CUSTOMER TESTIMONIALS        

"Our iPSC culture on LN521 is easy and stable. The use of LN521 allows us to maintain the iPSCs in monolayer culture for a prolonged time and also supports the differentiation of MKs and PLTs. It represents a good alternative to other matrices especially as it is fully defined and therefore compatible with GMP standards

Dorothee Eicke, PhD-Student, Hannover Medical School, Germany

"Before BioLamina, it was challenging to assess the reactivity of individual laminin isoforms towards cells because it is very difficult to purify laminins from tissues. Using the recombinant and very pure laminins from BioLamina, we could show that vascular isoforms containing the α5 or α4 but not α2 chain support efficient blood platelet adhesion and activation under flow in a process dependent on integrin α6β1, GPVI and the GPIb-VWF axis."

Dr. Mathieu Schaff, INSERM, Université de Strasbourg, France


         
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ARTICLES

HOW TO CULTURE HEMATOPOIETIC CELLS ON LAMININ SUBSTRATES

Robust and reliable HSC and blood cell maintenance and differentiation on defined and xeno-free human recombinant laminin-521 cell culture reagentsLaminins play a major role in the hematopoietic microenvironment

Laminins are widely expressed in human bone marrow play a major role in the hematopoietic microenvironment. Laminin isoforms 521, 511, 411 and 421 are the most abundant, synthesized by human bone marrow stromal cells (Siler, 2000). Laminin 521 and laminin-511 have been shown to have the strong adhesive interaction with CD34+ human hematopoietic stem cells (HSC) and progenitor cells, mediated largely via the integrin a6B1 receptor (Siler, 2000; Qian, 2006). Laminin-521 and laminin-511 is also highly adhesive to lineage-committed myelomonocytic and erythroid progenitor cells and most lymphoid and myeloid cell lines (Gu, 2003). These alpha-5 containing laminins also stimulate migration and proliferation of human hematopoietic progenitor cells (Gu, 2003), suggesting a important physiological role of laminins during hematopoiesis. Other laminin isoforms, such as laminin-111, show only weak adhesive interaction (Siler, 2000; Gu, 2003). Rodent CD34+ HSC transplantation experiment has shown that the laminin-integrin interaction also mediates homing (Qian, 2006; Carvalho, 2008).

 

Laminin-521 is an optimal substrate for clinically compliant hematopoietic differentiation

Laminin supports differentiation of megakryocytes and plateletsDue to the biorelevant environment created, laminin-521 is an optimal substrate for clinically compliant hematopoietic differentiation. In a publication by Börger et al, the authors present a xeno-free and defined protocol for the generation of functional megakaryocytes and platelets from a HLA-universal iPSCs on the human recombinant laminin-521 culture substrate (Börger, 2015). Laminins also has an important contribution to thrombus formation in vivo. Schaff and colleagues (2013) show that laminin-411, laminin-511, and laminin-521, but not laminin-211, allow efficient platelet adhesion and activation (via integrin a6B1) across a wide range of arterial wall shear rates.

iPS cell derived megakaryocyte with proplatelets.
Courtesy of Dorothee Eicke, Hannover Med. School.


A recent publication by Singapore researchers (Sivalingam, 2016) show that a laminin-521 coated microcarrier-based embryoid body (EB) platform support superior red blood cell (RBS) generation from human pluripotent stem cells. The authors developed a scalable, serum-free, xeno-free, and chemically defined microcarrier-based platform using human recombinant laminin-521 as an extracellular matrix for hPSC expansion, EB formation, and subsequently hematopoietic differentiation of hPSC. Improved survival and better quality EBs generated with the microcarrier-based method resulted in significantly improved mesoderm induction and resulted in drastic improvement in hematopoietic precursor expansion and the yield of RBS generation compared to a conventional EB-based differentiation method.

LAMININ KEY ADVANTAGES

  • Defined and xeno-free cell culture matrix for clinical compliance

  • Laminins play a major role in the hematopoietic microenvironment

  • Laminin-521, -511, -411 and -421 are the most abundant isoforms in bone marrow

  • A biologically relevant culture environment for HSC

  • Laminin-521 is an optimal substrate for clinically compliant hematopoietic differentiation, i.e. megakaryocytes, platelets and red blood cells

  • No lot-to-lot variability for standardized experiments with less variation

  • Scientifically validated in high-impact journals

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  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.