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LAMININ-521


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PROTOCOL

A simple, reproducible model of quiescent glomerular endothelial cell monolayer


CUSTOMER TESTIMONIAL 

HOW TO CULTURE RENAL CELLS ON LAMININ SUBSTRATES

Renal cell culture applications on human recombinant laminin substratesLaminins play an important role in kidney development

Both the tubular and glomerular epithelial cells of the kidney develop from mesenchymal stem cells during embryogenesis. A unique conversion of nonpolar cells to polarized epithelial cells thus occurs in the embryonic kidney. Organ cultures of mesenchymal cells from the mouse embryonic kidney have shown that laminins are particularly important for epithelial cell development (Ekblom, 1991).

Laminin 521 is the major laminin expressed in the mature glomerular basement membrane (GBM). During glomerulogenesis there are developmental transitions in laminin trimer deposition (St John and Abrahamson, 2001). Initially the GBM contains laminin 111, 411 and 511. By the capillary loop stage during GMB maturation, laminin 111 is eliminated from the GBM and the beta 2 chain begins to accumulate while the beta 1 chain is down regulated. At maturity, only laminin 521 is detected in the GBM.

 

A simple, reproducible and defined glomerular basement membrane filtration model on Biolaminin 521

Primary glomerular endothelial cells (GEnCs) is an important tool for studying glomerulosclerotic mechanisms and in drug development. In a publication by researchers at Novo Nordisk A/S, the authors establish a simple, reproducible and defined in vitro model of quiescent hGEnC monolayer on recombinant human Biolaminin 521 LN (Pajęcka, 2017). Biolaminin 521 best supported hGEnC attachment and spreading and culture on laminin resulted in a physiologically relevant barrier to 70 kDa dextrans which was 82 % tighter than that formed on collagen type IV. Furthermore, only hGEnCs cultured on laminin showed plasma-membrane localized zonula occludens-1 and vascular endothelial cadherin indicative of proper tight and adherens junctions. Biolaminin 521 culture promotes all the characteristics of the quiescent hGEnC monolayer: cobblestone morphology, well-defined adherens junctions and physiological perm-selectivity.


Laminin alpha-5 expressed in the mature glomerular basement membrane support culture of podocytes

The a-5 chain laminins (laminin 521 and laminin 511) binds predominantly to the integrin α3β1 receptor on the basal surface of podocytes. The binding of laminin 521 and/or laminin 511 to this integrin is essential for the formation of the typical glomerular capillary loop structure. Lack of binding to the α3β1 receptor results in severe kidney glomerular defects during development (Kikkawa, 2003). In a Nature protocols publication, reserchers from professor Inger's group at Harvard University have developed a detailed protocol for the directed differentiation of human iPS cells into mature, post-mitotic kidney glomerular podocytes with high (>90%) efficiency within 26 d and under chemically defined conditions, without genetic manipulations or subpopulation selection (Musah 2018). Biolaminin 511 LN (LN511) was used as cell culture substrate. The autors also describe how these iPS cell–derived podocytes may be induced to form within a microfluidic organ-on-a-chip culture device to build a human kidney Glomerulus Chip that mimics the structure and function of the kidney glomerular capillary wall in vitro. 


Mesangial cells produce laminin 421

Mesangial cells produce laminin 421 and organize glomerular capillaries by binding the laminin α-5 via α3β1 integrin and Lutheran receptor (Kikkawa, 2003). Thus, mesangial cells are important for the development and reparation of microvasculature and glomerulogenesis in general (Abrass, 2010). For culture of mesangial cells, we recommend Biolaminin 421.

LAMININ KEY ADVANTAGES

  • Biolaminin 521 enables a simple, reproducible and defined glomerular basement membrane filtration model

  • Biolaminin 521 promotes all of the characteristics of the quiescent hGEnC monolayer: cobblestone morphology, well-defined adherens junctions and physiological perm-selectivity

  • Culture on laminin resulted in a physiologically relevant barrier

  • High hGEnC attachment and spreading on laminin

  • Biolaminin 521 and 511 support differentiation and culture of podocytes

  • Defined and animal component-free culture method for reproduceble experments
  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Biolaminin 521 MX research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.