Laminin-121

LN121
Human recombinant laminin-121

THIS PRODUCTS IS CURRENTLY ONLY AVAILABLE AS PROTOTYPE MATERIAL


Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific.

Laminins-111/121 are widely expressed during embryogenesis, important for early epithelial development and differentiation of the epiblast thus vital for the development of an embryo However, the tissue distribution after birth is restricted to only a few tissues, such as brain and kidney. 

Laminin-121 can be used as a general attachment protein for most cell types in vitro and in particular for hepatic and neural differentiation, and to enhance neurite outgrowth. 

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Price
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100µg 1 x 1ml 0
1mg 10 x 1ml 0
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1-5 working days

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SIZE GUIDE

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

FORMAT
CONC. (ug/cm2).
NO OF WELLS
AMOUNT (uq)

Laminin-121 can support the survival, proliferation and differentiation of many different cell types in vitro, although many of those cultivated cell types would not encounter the protein naturally in vivo. Instead you should match your specific cell type to the laminin naturally expressed by this cell which leads to improved cell culture.

In the adult, laminin α1 chain expression is relatively restricted and is only present in some epithelial basement membranes, and primarily found in tissues such as the eye, liver and kidney, whereas hardly expressed at all in endothelial, adipose, nervous, and muscle tissues (Ekblom, 2003; Virtanen, 2000). 

 

Commonly, laminin-111 have been used as a general attachment protein for most cell types in vitro. Laminin-121 has not been commercially available until now and therefore the functional comparison between LN121, LN111 and other laminins isoforms is yet to be performed in order to understand the potential for LN121 for cells in culture.

However, known is that the laminin beta 2 chain (LN121) has been suggested to have a higher affinity for integrins compared to the alfa 1 chain (LN111) thus influencing stability of intergin-laminin and activation of downstream effectors in cell signaling cascades.

 

It is unknown why laminin-121 can exert all these major biological effects on such a wide selection of cell types, but one explanation is that, depending on the cell type, laminin-121 can not only induce different cell signaling cascades but as all laminins also co-signal with various growth factors.

However, by coating your plate with recombinant laminin-121 you support your cells with a defined and xeno-free substrate. Therefore, human recombinant laminin-121 enables better control of your cell matrix and experiments.

 

COATING PROCEDURE

1. Slowly thaw recombinant laminins at +4°C before use. Thawed laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions. For your convenience, the coated plates and diluted coating solution can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted Laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spreaded evenly across the surface. Note that the laminin matrix will be inactivated if let dry.

IMPORTANT NOTES

  • The Laminin stock solution is stable for 2 years when stored at -20°C. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C. Repeated freeze thawing should be avoided. 

  • The protocol can easily be made totally defined and xeno-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

  • Coat the wells in advance with the laminin solution according to the instruction here

Product name

LN121

 

Product use

Cell culture

 

Product application

General cell culture substrate


 

Documents

MSDS

Storage

-20°C

 

Stability

Three years from date of receipt

 

Stock concentration

0.1mg/ml

 

Use concentration

0.5-2 ug/cm2
Optimal concentration must be determined by the end user

Protein name

Laminin-121

 

Synonyms

Laminin-3

 

Expressed in vivo

Expressed and secreted by trophectoderm and some epithelial basement membranes. Can thus be found in the placenta, skeletal muscles and kidneys.