Cell-specific culture matrices for improved cell functionality

BioLamina offer an expansive portfolio of recombinant laminin proteins for a variety of applications, including reliable expansion of human pluripotent stem cells (hPSCs) and differentiation and maintenance of different specialized cell types.  
 Buy human recombinant laminin cell culture matrices here

  • Chemically defined and xeno-free cell culture matrices

  • Allow you to imitate the natural cell-matrix interactions in vitro, leading to improved cell functionality

  • Cell-specific culture environment

  • No lot-to-lot variability

  • Flexible culture system

  • Technology scientifically proven in high-impact journals    

 

LAMININ CELL CULTURE APPLICATIONS 

Click on the icons below to read more about different cell culture applications for laminins

 

stem cell culture and on biorelevant cell culture substrates

HAIR FOLLICLE CELLS
& SKIN CELLS

RETINAL CELLS, CORNEAL CELLS
& PHOTORECEPTORS

THYMOCYTES
& IMMUNE CELLS

SKELETAL MUSCLE CELLS

HEPATOCYTES & HEPATOBLAST CELLS

PANCREATIC ISLETS
& BETA CELLS

ADIPOSE CELLS

hPSC DERIVATION AND EXPANSION 
iPSC REPROGRAMMING AND EXPANSION

CLONAL STEM CELL CULTURE

NEURONSGLIA
& NEURAL STEM CELLS

MAMMARY GLAND CELLS

LUNG CELLS

CARDIOMYOCYTES

SPLEEN CELLS

ENDOTHELIAL CELLS

HEMATOPOIETIC STEM CELLS
& BLOOD CELLS

KIDNEY CELLS

INTESTINAL CELLS

GERM CELLS

MESENCHYMAL STEM CELLS

BONE 
& CARTILAGE CELLS 

CANCER CELLS 

                                                                                                        DOWNLOAD THE LAMININ CELL CULTURE APPLICATIONS POSTER


The laminin-521 stem cell matrix

natural stem cell culture substrate
In the developing embryo, laminins are among the first extracellular proteins to be expressed. Laminin-521 is a key cell adhesion protein of the natural stem cell niche, expressed and secreted by hPSCs in the inner cell mass of the embryo. Laminin-521 is also produced endogenously by pluripotent stem cells cultured in vitro and are critical autocrine and paracrine factor that regulates their survival and self-renewal.


Laminin-521 successfully recreates the biologically relevant hPSC milieu in vitro and via integrin binding, laminin-521 induces the PI3K/Akt signaling pathway, promoting high survival and robust long-term self-renewal of human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC). The cells grow in a homogeneous monolayer with maintained pluripotency and genetic integrity and there is no need for manual removal of differentiated cell areas. With the defined and xeno-free laminin-521 cell culture system culture of hPSC becomes easy, reliable and standardized. 

 


KEY BENEFITS OF LAMININ-521 FOR HUMAN PSC CULTURE

  • Defined and xeno-free cell culture matrix - for controlled cell culture and clinical compliance

  • No lot-to-lot variability - more reliable and standardized experiments

  • Homogenous hPSC cultures that are pluripotency and genetically stabile - no need to remove differentiated cell areas

  • High clonal survival - optimal substrate for derivation and gene editing 

  • Triggers authentic cell signaling pathways which generates hPSCs cultures with a more uniform gene expression profile

  • High hPSCs expansion rate and controlled single-cell passaging - facilitates automation and high-throughput processes

  • Authentic milieu that primes the hPSCs which results in more efficient differentiation and enhanced cell maturation, polarization and organization

  • Flexible culture system - easy to control and adaptable to any protocol

  • Support weekend feeding - no more weekend work!

     

Download the application note for hPSC culture on the laminin-521 stem cell matrix.

  

 

FASTER EXPANSION OF hPSCs ON LAMININ-521

rapid stem cell expansion hESC and iPSC grow faster on laminin-521 (LN-521) compared to other feeder-free matrices, such as Vitronectin and EHS extracts (e.g. Matrigel), facilitating automation. The specific binding of LN-521 to the α6β1 integrin enables high hPSC survival and self-renewal. PSCs on LN-521 grow as a homogenous monolayer without any abnormal genetic aberrations. Once adapted to the LN-521 matrix, hPSCs can routinely be cultured as single cells without the addition of apoptosis inhibitors, such as ROCKi. LN-521 also support high clonal survival and is a good substrate for derivation and gene editing.

pluripotent stem cells culture without spontaneous differentiation

NO SPONTANEOUS DIFFERENTIATION 

Compared to laminin-521 (LN-521), many other feeder-free matrices, such as extracts of the Engelbreth-Holm-Swarm (EHS) tumor, cannot provide the correct biorelevant signals to support hPSCs which often results in loss of pluripotency and genetically drift in vitro. Human PSCs can be cultured to near confluence on LN-521, without risk of spontaneous differentiation. The cells remain pluripotent (Oct4+; pink) on LN-521 and there is no need to remove differentiated cell areas (DAPI staining only; blue) as compared to cells cultured on other substrates. 

 

 Cost effective hPSC culture

COST-EFFECTIVE HUMAN PSC EXPANSION 

Due to faster growth rate and higher cell yield, the total cost per cell for an average passage is lowest for laminin-521 (LN-521), compared to other feeder-free matrices. To minimize cost, simply adjust the culture ware size to fit your need.


Read more about how laminin-521 saves both time and money for cost-effective pluripotent stem cell culture.

 

 

WEEKEND-FREE FEEDING

For reduced labor and cost, the laminin-521 matrix supports culture of human PSCs without weekend-free feeding. The protocol is flexible - just feed your cells daily during weekdays and passage your cells on Thursday or Friday. Your hPSC cultures will maintain characteristic cell morphology, high pluripotent cell marker expression and high expansion rates. Read more about weekend-free hPSC culture protocol.


Download the weekend-free feeding application note
. 

  


EASY AND FLEXIBLE CULTURE PROTOCOLSeasy and flexible stem cell culture

When using laminin-521, your cell culture protocol can easily be made totally defined and xeno-free, with your choice of culture medium and dissociation reagent.

  1. Coat plates with laminin.

  2. Wash the cells with PBS and add dissociation reagent of choice - incubate

  3. Centrifuge the single-cell suspension and resuspend the pellet in fresh medium of choice

  4. Seed as single-cell suspension on fresh laminin coated plates

 

Download the coating protocol and the protocol for how to culture hPSCs on laminin-521.
 

 stem cell culture protocol

 

We have visualized the article Monolayer culturing and cloning of human pluripotent stem cells on laminin-521–based matrices under xeno-free and chemically defined conditions by Rodin et al., published in Nature Protocols 2014. This is a quick guide and overview of how to utilize laminin-521 as your matrix of choice for your hPSCs through the complete stem cell propagation process.


Here you can order the A1 size hPSC culture poster or download the PDF HERE.

 

RECOMMENDED PUBLICATIONS

 

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.