Li J., Tzu J., Chen Y., Zhang Y.P., Nguyen N.T., Gao J., Bradley M., Keene D.R., Oro A.E., Miner J.H., Marinkovich M.P.
EMBO J., 2003
Here, the authors describe the essential role of laminin-511 in hair follicle development. Treatment of human scalp xenografts with antibodies to laminin-10, or its receptor β1 integrin, produced alopecia. Skin from lama5-null mice fails to support hair development. Absence of laminin-511 in transgenic mice led to a number of developmental abnormalities, including arrest of hair development and deficient Shh expression in hair follicles. Transplantation of skin grafts from these Lama5-null mouse embryos onto healthy mice failed to support hair growth. Intriguingly, purified laminin-511 (isolated from A549 lung carcinoma cell conditioned medium) was able to restore hair development in the Lama5-null skin. The authors conclude that laminin-511 is required for hair follicle development and report the first use of exogenous protein to correct a cutaneous developmental defect.
Sugawara K., Tsuruta D., Kobayashi H., Ikeda K., Hopkinson S.B., Jones J.C., Ishii M.
J Histochem Cytochem., 2007
Here they characterized changes in laminin isoform expression during hair cycling. At the mRNA level, laminin-511 expression undergo a steady increase during anagen stages. In contrast, laminin-332 expression is initially upregulated in outer root sheath (ORS) keratinocytes at anagen II and then transiently downregulated. Immunohistochemistry demonstrated that laminin-332 and α6β4 integrin were well colocalized, but their signals were remarkably decreased in the lower half of follicles after anagen VI. In hair follicle culture, laminin-511/521-rich human placental laminin enhanced hair growth, whereas recombinant laminin-332 antagonized hair growth induced by laminin-511. Our results indicate a positive role for laminin-511 and a negative role for laminin-332 on hair growth.
Gao J., DeRouen M.C., Chen C.H., Nguyen M., Nguyen N.T., Ido H., Harada K., Sekiguchi K., Morgan B.A., Miner J.H., Oro A.E., Marinkovich M.P.
Genes Dev., 2008.
Here the authors demonstrate the mechanism of how laminin-511 controls hair morphogenesis. Dermal papilla (DP) from laminin-511 mutants showed developmental defects by E16.5 in mice, including a failure to maintain expression of the key morphogen noggin. Laminin-511 mutant DP showed decreased length and structure of primary cilia in vitro and in vivo. Laminin-511, but not laminin-111, restored primary cilia formation in lama5−/− mesenchyme and triggered noggin expression in an Shh- and PDGF-dependent manner. Hair development required the B1 integrin binding but not the heparin binding domain of laminin-511. Inhibition of laminin-511 receptor B1 integrin disrupted DP primary cilia formation as well as hair development. These studies show that epithelial-derived laminin-511 is a critical early signal that directs ciliary function and DP maintenance as a requirement for hair follicle downgrowth.
DeRouen M.C., Zhen H., Tan S.H., Williams S., Marinkovich M.P., Oro A.E.
BMC Dev Biol., 2010
With the use of a basal cell carcinoma (BCC) model system and mouse mutants the authors re-evaluate the role of laminin-511 in epithelial invagination in the skin. Blocking laminin 511 and 332 in BCCs maintains primary cilia and Shh signalling, but prevents invagination. Similarly, in laminin-511 and dermal beta-1 integrin mutants, dermal papilla development and primary cilia formation are normal. Dermal beta-1 integrin mutants have normal hair follicle development. They conclude that laminin-511 has a primary role of promoting hair follicle epithelial downgrowth without affecting dermal primary cilia and Shh target gene induction.
Lay K., Kumeb T., Fuchsa E.
Here, the authors suggest that hair follicle stem cells (HFSCs) have restricted potential in vivo, which they conserve by coupling quiescence to adhesion-mediated niche maintenance, thereby achieving long-term tissue homeostasis. They examine whether parsimonious stem cells use is essential to conserve long-term tissue-regenerating potential during normal homeostasis by conditionally ablating a key transcription factor Forkhead box C1 (FOXC1). FOXC1-deficient HFSCs spend less time in quiescence, leading to markedly shortened resting periods between hair cycles. The enhanced hair cycling accelerates HFSC expenditure, and impacts hair regeneration in aging mice. Interestingly, although FOXC1-deficient HFs can still form a new bulge that houses HFSCs for the next hair cycle, the older bulge is left unanchored. Hair follicle stem cells lacking the protein FOXC1 can only retain one old bulge in their hair follicles, while normal stem cells can keep up to four. In vitro cell adhesion assay they show that FACS-purified WT and Foxc1-KO HFSCs attach very well to LN-511 with about 6 times higher well area coverage compared to collagen I, fibronectin or Matrigel-coated plates.
Pouliot N., Saunders N.A., Kaur P.
Experimental Dermatology, 2002
They investigated the expression and function of the laminin-511 and -521 in neonatal and adult human skin. These isoforms are expressed abundantly in the basement membrane underlying the inter-follicular epidermis and the blood vessels in the dermis. The expression levels did not change significantly with age, but appeared to decrease in the basement membrane underlying the epidermis, in adult skin. In contrast, the levels of laminin-511 and -521 in the basement membrane underlying blood vessels remained unchanged in neonatal vs. adult skin. An in vitro cell adhesion assays demonstrated that laminin-511 and -521 is potent adhesive substrates for both neonatal and adult keratinocytes and that this adhesion is mediated by the a3b1 and a6b4 integrins. Further, laminin-511 and -521 provide a proliferative signal for neonatal foreskin keratinocytes, adult breast skin keratinocytes, and even a human papillomavirus type-18 transformed tumorigenic keratinocyte cell line in vitro. Laminin-511 and -521 also stimulate keratinocyte migration in an in vitro wound healing assay. These results provide strong evidence for a functional role for laminin-10/11 in epidermal proliferation during homeostasis, wound healing and neoplasia.
Morgner J., Ghatak S., Jakobi T., Dieterich C., Aumailley M., Wickström S.A.
Nat Commun., 2015
In the present study the authors conclude that the precise ratio between LN-332 and LN-511 adjusts activities of key signalling pathways that determine SC activation within the hair follicle bulge niche. They suggest that integrin-linked kinase (ILK) is required for the maintenance of quiescent bulge SCs through remodelling of the ECM niche, thereby governing the activation and maintenance of hair follicle stem cells (HFSCs). ILK mediates deposition of inverse laminin-332 and -511 gradients within the basement membrane (BM) wrapping the hair follicles. The precise ratio of LN-511 and LN-332 regulates core SC fate-determining signalling pathways and that this ratio is disturbed in the absence of ILK, leading to aberrant SC activation and failure to re-establish quiescence. The BM composition tunes activities of Wnt and transforming growth factor-β pathways and subsequently regulates HFSC activation. LN-511, present at low levels around the bulge and at higher levels around the hair germ/TACs, promotes Tgf-β signalling, whereas LN-332, highly expressed along the IFE and to a lesser extent along the upper regions of HFs, suppresses Wnt/β-catenin signalling. Notably, reconstituting an optimal laminin microenvironment restores the altered signalling in ILK-deficient cells.
Kariya Y., Sato H., Katou N., Kariya Y., Miyazaki K.
PLOS ONE, 2012
Laminin-332 is known to supports the stable anchoring of basal keratinocytes to the epidermal basement membrane but is also motility factor for wound healing and cancer invasion. Here they investigated Laminin-332 matrices deposited by normal human keratinocytes and several cancer cell lines. All types of the cells efficiently deposited Laminin-332 on the culture plates in specific patterns. On the contrary, laminins containing laminin ß1 and/or c1 chains (such as Lm511 and Lm311) were not deposited on the culture plates even if secreted into culture medium. The deposited Laminin-332 matrix showed a mesh-like network structure as analyzed by electron microscopy, suggesting that Lm332 was highly polymerized. Laminin-332 matrix rather suppressed the migration of keratinocytes as compared with purified Lm332 (not a BioLamina product), which highly promoted the cell migration. The Lm332 matrix supported adhesion of keratinocytes much more strongly and stably than purified Lm332. Integrin a3ß1 bound to the Lm332 matrix at a three times higher level than purified Lm332. These results indicate that the polymerized Lm332 matrix supports stable cell adhesion whereas unassembled soluble Lm332 supports cell migration. The question is though how the purified Ln-332 looked like. Difficult to purify and might be fractionated.
Steffens D., Mathor M.B., Santi B.T.S., Luco D.P., Pranke P.
Regen. Med., 2015
Here they developed s scaffolds of poly-DL-lactic acid with and without the linkage of laminin-332, bringing together MSCs and keratinocytes aimed for treatment as a new skin substitute. Three groups of scaffolds were studied: 1) poly-DL-lactic acid (PDLLA), 2) hydrolyzed PDLLA (PDLLA/NaOH) and 3) PDLLA/Lam which is a PDLLA/NaOH scaffold linked to laminin-332. The results corroborate the hypothesis that laminin influenced the adhesion of the MSCs. Laminin significantly promoted the adhesion and spreading of proliferating oral and epidermal keratinocytes compared with collagen nanofibers only. The use of biocompatible and biodegradable polymers associated with the properties of laminin lead to an improvement in the adherence and viability of the cells, showing LN-332 is beneficial for growth of MSCs and keratinocytes.
Pouliot N., Saunders N.A., Kaur P.
Exp Dermatol., 2002
The authors investigated the expression and function of laminin-511 and -521 in neonatal and adult human skin. They found that the laminin-a5 chain is expressed abundantly in the basement membrane underlying the interfollicular epidermis and the blood vessels in the dermis. Interestingly, while the expression level of the well-studied laminin-5 isoform did not change significantly with age, laminin-511 and -521 appeared to decrease in the basement membrane underlying the epidermis, in adult skin. In contrast, the levels of laminin-511 and -521 in the basement membrane underlying blood vessels remained unchanged in neonatal vs. adult skin.
Hyejung Jung and Eok-Soo Oh
Pigment Cell & Melanoma Research, 2016
Here they investigate the mechanism through which FK506 regulates migration of melanocytes. B16F10 melanoma cells were cultured on laminin-332, an optimal substrate for regulating the adhesion and migration of melanocytes and melanoma cells. FK506 treatment enhanced cell spreading on laminin-332 and increased migration in both melanocytes and melanoma cells.
Ritié L., Spenlé C., Lacroute J.I., Bolcato-Bellemin A-L., Lefebvre O., Bole-Feysot C., Jost B., Klein A., Arnold C., Kedinger M., Bagnard D., Orend G., Simon-Assmann P.
PLOS ONE, 2012
Laminin-511 is highly expressed in the intestine. To understand the mechanistic role of laminin-511 in tissue homeostasis, the researchers used RNA profiling of embryonic intestinal tissue of lama5 knockout mice and identified a lama5 specific gene expression signature. They show that laminin a5 plays a crucial role in both epithelial and mesenchymal (smooth muscle) cell behavior by inhibiting Wnt and activating PI3K signaling. We conclude that conflicting signals are elicited in the absence of lama5, which alter cell adhesion, migration as well as epithelial and muscle differentiation. The LMa5 deficient intestine also displays a smooth muscle defect and myogenic differentiation markers are affected. Laminin-511 supports adhesion of epithelial cells and Akt phosphorylation. Laminin-511 stimulates spreading of epithelial and muscle cells (compared to laminin-111). Inhibition of Akt with wortmannin abolished spreading of epithelial cells on laminin-511 as evidenced by cell laminin-511 specifically activates Akt through the PI3K pathway in intestinal epithelial but not in mesenchymal cells. Cell migration was also higher on Laminin-511. Laminin-511 also protects cells against H2O2-induced apoptosis.
Teller I.C., Auclair J., Herring E., Gauthier R., Ménard D., Beaulieu J-F.
Developmental dynamics, 2007
Here, the expression of the five laminin a-chains was analyzed in the developing and mature human small intestine at the protein and transcript levels in order to further delineate specific involvement of individual laminins in relation to the epithelial cell state as defined along the functional crypt-villus axis. The results show that all of the a-laminins are expressed in significant amounts in the small intestine relative to a panel of other tissues and organs. Distinct epithelial and mesenchymal origins, as well as differential occurrence in intestinal basement membranes according to developmental stage, along the crypt-villus axis and in compartment-related experimental intestinal cell models. Taken together, the data point out the prime importance of a2-, a3-, and a5-containing laminins for the development and maintenance of the functional human intestinal epithelium.
Mahoney Z.X., Stappenbeck T.S., Miner J.H
J Cell Sci. 2008
The villus basement membrane is rich in laminin α5. Here the authors show that diminution of laminin α5 in a mouse model led to a compensatory deposition of colonic laminins that resulted in a transformation from a small intestinal to a colonic mucosal architecture. The alteration in mucosal architecture was associated with reduced levels of nuclear p27Kip1, a cell cycle regulator, and altered intestinal epithelial cell proliferation, migration, and differentiation. The results suggest that laminin α5 plays a crucial role in establishing and maintaining the specific mucosal pattern of the mouse small intestine.
Bolcato-Bellemin A-L., Lefebvre O., Arnold C., Sorokin L., Miner J. H., Kedinger M., Simon-Assmann P.
Developmental Biology, 2003
Here, the function of the laminin a5 chain in the developing intestine was defined by analysing laminin a5 -/- mutants and by grafting experiments. The authors show that laminin a5 plays a major role in smooth muscle organisation and differentiation, as excessive folding of intestinal loops and delay in the expression of specific markers are observed in laminin a5 -/- mice. Loss of a5 expression was paralleled by ectopic or accelerated deposition of laminin a2 and a4 chains; this may explain why no obvious defects were observed in the villous form and enterocytic differentiation. Lack of the laminin a5 chain was accompanied by a decrease in epithelial a3B1 integrin receptor expression adjacent to the epithelial basement membrane and of Lutheran blood group glycoprotein in the smooth muscle cells, indicating that these receptors are likely mediating the a5 interactions. Taken together, the laminin a5 chain is essential for normal development of the intestinal smooth muscle.
Lefebvre O., Sorokin L., Kedinger M., Simon-Assmann P.
Developmental Biology, 1999
In this study, the authors examine the expression patterns and the cellular origins of the laminin a2, a4, and a5 chains in the developing mouse intestine and in in vitro mouse/chick or chick/mouse interspecies hybrid intestines. All three laminin a chains are highest in the fetal intestine undergoing intense morphogenetic movements. Laminin a4 are associated with mesenchyme-derived cell populations such as endothelium and smooth muscle. In contrast, laminin a2 and a5 chains participate in the structural organization of the subepithelial basement membrane and, in the mature intestine, show a complementary pattern of expression. All three laminins chains occur in the smooth muscle basement membrane. Laminin a2 was found to be deposited into the basement membrane exclusively by mesenchymal cells, and the laminin a5 chain was deposited by both epithelial and mesenchymal cells in an apparently developmentally regulated pattern.