PRODUCT RECOMMENDATION

Early intestinal differentiation:
LAMININ-111

Intestinal maturation:
LAMININ-511


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HOW TO CULTURE INTESTINAL CELLS ON LAMININ SUBSTRATES

HOW TO CULTURE INTESTINAL CELLS ON LAMININ SUBSTRATESLaminin expression in the intestine

The small intestine contains mucosal epithelial invaginations called crypts of Lieberkühn that are continuous with evaginations into the lumen called villi. The intestinal epithelia are self-renewed by a population intestinal stem cells within the intestinal crypt that give rise to progenitor cells, which can subsequently differentiate into the mature cell types. The intestinal epithelium is in direct contact with a basement membrane. All laminin a-chains (laminin-111, -211, -332, -411 and -511) are expressed at significant amounts in the small intestine. Importantly, they are distributed in specific patterns along the crypt-villus axis of the intestine and are developmentally regulated (Teller, 2007; Lefebvre, 1999). 


Laminin-111 have a vital role in the early developing intestine

The α1 laminin expression is restricted to the intervillous areas in the early developing intestine and is gradually replaced by the α2 laminins as crypts begin to form. The α3 and α5 laminins were both expressed at the base of the intestinal epithelium at early stages of gut development but tend to be restricted to the villus from mid-gestation onward. The α4 laminins is not expressed in epithelial basement membrane (Teller, 2007).


Alpha-5 laminins are crucial for establishing and maintaining the intestinal architecture

The villus basement membrane is rich in laminin α5 which is crucial for both establishing and maintaining the small intestinal crypt-villus architecture (Mahoney, 2008; Ritié, 2011). In a publication by Ritié et al., the authors describe a mechanistic link between laminin α5 gene deficiency and the physiological phenotype showing that laminin a5 plays a crucial role in both epithelial and mesenchymal cell behaviour by regulating Wnt and PI3K signaling (Ritié, 2011). It's concluded that laminin-511 stimulates expression and activity of the survival factor Akt and stimulate cell adhesion, migration as well as epithelial differentiation (Ritié, 2011). In the absence of laminin α5, the proliferative compartment of the intestine expands, suggesting a delay in initiating differentiation (Mahoney, 2008). Lack of the laminin α5 chain was accompanied by a decrease in epithelial α3β1 integrin and the Lutheran receptor, indicating that those are likely targets for the laminin α5 laminins (Bolcato-Bellemin, 2003). The B4 integrin is also expressed in the Enterocytes differentiated normally in the absence of laminin α5 but the terminal differentiation of goblet cells was affected, as shown by the increased numbers of intermediate cells and alteration of mucous granules towards the colon type (Mahoney, 2008). The α5 laminins has also shown to a major role in intestinal smooth muscle organization and differentiation (Bolcato-Bellemin, 2003).

We recommend laminin-111 for early intestinal specification and laminin-511 for maturation and maintenance.

LAMININ KEY ADVANTAGES

  • All laminin a-chains (laminin-111, -211, -332, -411 and -511) are expressed at significant amounts in the small intestine.

  • Different laminin isoforms are distributed in specific patterns along the crypt-villus axis of the intestine and are developmentally regulated. 

  • Laminin-111 expression is restricted to the intervillous areas in the early developing intestine.

  • The α3 and α5 laminins are both expressed at the base of the intestinal epithelium at early stages of gut development.

  • The villus basement membrane is rich in laminin α5, crucial for both establishing and maintaining the small intestinal crypt-villus architecture.

  • Laminin a5 plays a crucial role in both epithelial and mesenchymal cell behavior by regulating Wnt and PI3K signaling.
  • Defined and xeno-free cell culture matrix for clinical compliance

  • No lot-to-lot variability for standardized experiments with less variation

  • Scientifically validated in high-impact journals

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.