Biolaminin 221 LN

Biolaminin 221 LN (LN221)

Human recombinant laminin 221


Recreating the natural cell niche is key to succesfull cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific. 

Human recombinant laminin 221 substrate, Biolaminin 221 LN (LN221), supports the growth, survival and differentiation of a wide range of tissue-specific cell types, including cardiac cells and skeletal muscle cells.

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Amount
Size
Price
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100 µg 1 x 1 ml €71
1 mg 10 x 1 ml €625
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Estimated shipping time
1-5 working days

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SIZE GUIDE

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

FORMAT
CONC. (ug/cm2).
NO OF WELLS
AMOUNT (uq)

Laminin 221 is important for muscle development and function and is togheter with laminin 211 one of the main lamionin isoforms present in adult muscle tissue, including varying amounts of laminin 521 and laminin 421 depending on tissue.

Mutations of the LAMA2 gene are the most common cause of congenital muscular dystrophy that frequently leads to death in early childhood (Domogatskaya, 2012). For a review on α2-laminin in skeletal muscle function, see Holmberg and Durbeej, 2012.

Laminin 211 as well as laminin 221, are very important for cardiomyocytes and heart muscle development. In the developing heart both laminin 211 and laminin 221 are expressed in the extracellular matrix of cardiomyocytes as well as in the basement membrane zones of the endo- and pericardium and the capillaries (Roediger, 2010).

Laminins containing the α2-chain (such as laminin 221) are expressed in the ventricular zone ECM in the developing mouse central nervous system. Since laminins are known to contribute to the stem cell niche in the brain, this suggests that laminin 221 could play an important functional role in the regulation of neural stem cells and progenitor cells (Haubst, 2006; Kazanis, 2010; Lathia, 2007; Mercier, 2002).

Authentic cardiomyocyte cell culture

Heart

Cardiomyocyte cell culture 

 

Muscle laminin

Muscle cells

Smooth and skeletal
muscle cell culture 

COATING PROCEDURE

1. Slowly thaw recombinant laminins at +4°C before use. Thawed laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions. For your convenience, the coated plates and diluted coating solution can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. Coat the wells in advance with the laminin solution according to the instruction here

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spreaded evenly across the surface. Note that the laminin matrix will be inactivated if let dry.

IMPORTANT NOTES

  • The Laminin stock solution is stable for 3 years when stored at -20°C to -80°C. Repeated freeze thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. 

  • The protocol can easily be made totally defined and xeno-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

  • Coat the wells in advance with the laminin solution according to the instruction here

Product name

Biolaminin 221 LN

 


Product code

LN221

 


Declaration

For research use only

 


Documents

MSDS

CoA

Storage

-20°C to -80°C

 


Stock concentration

0.1mg/ml

 


Appearance

Clear, colourless, buffered solution with a 
pH of 7.2 with 10% glycerol and 0.02% NaN3

 

Shipping Condition

Dry Ice

PROTEIN NAME

Laminin 221 (Laminin-4)

 


Classification

Defined and xeno-free, human 
recombinant protein 

 

Product application

Culture of skeletal and smooth muscle cells, cardiomyocytes, glia cells and neural progenitor cells