Laminin-421

LN421
Human recombinant laminin-421


Recreating the natural cell niche is key to succesfull cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific. 

Human recombinant laminin-421 supports several tissue-specific cell types such as endothelial cells and kidney cells, and has been suggested to be important for renal, synaptic and inflammatory functions.

 

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Amount
Size
Price
Oty
100µg 1 x 1ml €69
1mg 10 x 1ml €610
For larger quantities contact us

Estimated shipping time
1-5 working days

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SIZE GUIDE

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

FORMAT
CONC. (ug/cm2).
NO OF WELLS
AMOUNT (uq)

In the kidney, laminin-421 is produced by mesangial cells and is presumably important for the development and reparation of microvasculature and glomerulogenesis (Abrass, 2010) whereas kidney podocytes mainly bind to laminin-521 via the α3β1 integrin (Sachs and Sonnenberg, 2013). Laminin-421 has also been implicated in renal cell carcinomas (Lohi, 1996; Vainionpää, 2007) and glial brain tumours (Ljubimova, 2001).

In chemical synapses, the laminin-421 isoform is crucial for the correct localization of pre- and postsynaptic specializations. In mice lacking the laminin β2 chain, neuromuscular junctions fail to form active zones in the presynaptic terminal and thereby neurotransmitter release is disrupted (Patton, 2001). Laminin-421 has been shown to assist in the formation and stabilization of active zones by several mechanisms, including voltage-gated Ca2+-channels and integrins containing the α3 subunit (Carlsson, 2003; Cohen, 2000).

laminin endothelial cell culture

Endothelial cells

Maturation and maintenance
of blood vessels 

COATING PROCEDURE

1. Slowly thaw recombinant laminins at +4°C before use. Thawed laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions. For your convenience, the coated plates and diluted coating solution can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spreaded evenly across the surface. Note that the laminin matrix will be inactivated if let dry.

IMPORTANT NOTES

  • The Laminin stock solution is stable for 2 years when stored at -20°C. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C. Repeated freeze thawing should be avoided. 

  • The protocol can easily be made totally defined and xeno-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

  • Coat the wells in advance with the laminin solution according to the instruction here

Product name

LN421

 

Product use

Cell culture

 

Product application

Endothelial cells, mesangial cells and maturation of neuromuscular junctions


 

Documents

MSDS

Storage

-20°C

 

Stability

Three years from date of receipt

 

Stock concentration

0.1mg/ml

 

Use concentration

0.5-2 ug/cm2
Optimal concentration must be determined by the end user

Protein name

Laminin-421 (LN-421)

 

Synonyms

Laminin-9

 

Expressed in vivo

Expressed and secreted by mesangial cells, endothelial cells and can, in addition, also be found in the presynaptic terminal of neuromuscular junctions.