Biolaminin 421 LN

Biolaminin 421

Human recombinant laminin 421

Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue-specific. 

Human recombinant laminin 421, Biolaminin 421 LN (LN421), supports several tissue-specific cell types such as endothelial cells and kidney cells and has been suggested to be important for renal, synaptic and inflammatory functions.



100 µg 1 x 1 ml €78
500 µg 1 x 5 ml €339
For larger quantities contact us

Estimated shipping time
1-5 working days

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Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

CONC. (ug/cm2).

In the kidney, laminin 421 is produced by mesangial cells and is presumably important for the development and reparation of microvasculature and glomerulogenesis (Abrass, 2010) whereas kidney podocytes mainly bind to laminin 521 via the α3β1 integrin (Sachs and Sonnenberg, 2013). Laminin 421 has also been implicated in renal cell carcinomas (Lohi, 1996; Vainionpää, 2007) and glial brain tumors (Ljubimova, 2001).

In chemical synapses, the laminin 421 isoform is crucial for the correct localization of pre- and post-synaptic specializations. In mice lacking the laminin β2 chain, neuromuscular junctions fail to form active zones in the presynaptic terminal and thereby neurotransmitter release is disrupted (Patton, 2001). Laminin 421 has been shown to assist in the formation and stabilization of active zones by several mechanisms, including voltage-gated Ca2+-channels and integrins containing the α3 subunit (Carlsson, 2003; Cohen, 2000).

laminin endothelial cell culture

Endothelial cells

Maturation and maintenance
of blood vessels 


1. Slowly thaw recombinant laminins at +4°C before use. 

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. 

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spread evenly across the surface. Note that the laminin matrix will be inactivated if let dry.


  • The laminin stock solution is long-term stable when stored at -20°C to -80°C. Please refer to the product-specific CoA for shelf life details.

  • Repeated freeze-thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. Thawed, undiluted laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions.

  • Avoid long exposure of the protein to ambient temperatures.

  • For your convenience, the coated plates can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

  • The protocol can easily be made totally defined and animal origin-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

Product name

Biolaminin 421 LN


Product code




For research use only






-20°C to -80°C


Stock concentration




Clear, colorless, buffered solution with a 
pH of 7.2 with 10% glycerol and 0.02% NaN3


Shipping Condition

Dry Ice

Protein name

Laminin 421 (Laminin-9)



Defined and animal origin-free, human 
recombinant protein 


Product application

Culture of endothelial cells, mesangial cells, and maturation of neuromuscular junctions