Not sure which laminin isoform works best for your cells?
Now you can choose 4 different Biolaminin LN products (100 ug) and only pay for 3. This allows you to test which Biolaminin substrate is optimal for your cell culture.

Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue-specific.


400 µg 4 x 100 ug €219
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Robust cell culture is achieved by culturing cells on their natural cell adhesion molecule, laminin. All cells in the body make close contact with the extracellular matrix and one component, the basement membrane, surrounds all organs and functional units in vivo. The basement membrane is important for cell anchorage, survival, migration, differentiation, and function.

There are many proteins in the basement membrane, but laminins are tissue-specific and play major roles in creating and sustaining cellular niches.

To date, more than 16 different laminin isoforms have been described. Through studies of human diseases and gene deletion experiments, the functions of a few isoforms have been elucidated. However, the exact role for most isoforms as well as the composition of laminins in different cell niches is still unknown.

In order to maintain the proper function of cells and to differentiate them towards desired cell phenotypes, correct replication of the laminin foundation in the in vivo cell niche is essential.

LAMscreen makes it easy for you to test which Biolaminin(s) provides the best support for your cells in vitro.

The following laminin isoforms have been demonstrated to serve critical roles for the listed cell types:


Biolaminin 521 LN (LN521)

Single-cell human ES/iPS cell culture (Rodin, 2014)
Clonal pluripotent stem cell culture (Rodin, 2014)
Podocytes (Miner, 2011)


Biolaminin 511 LN (LN511)

Culture of human ES/iPS cells (Rodin, 2010)
RPE-cells culture (Aisenbrey, 2006)
Hair follicles (Li, 2003)
Certain neuronal cells (Fusaoka-Nishioka, 2011)


Biolaminin 421 LN (LN421)

Mesangial cell culture (Abrass, 2010)
Kidney microvasculature (Thyboll, 2002)
Motorneuron synaptic transmission (Patton, 2001)


Biolaminin 411 LN (LN411)

Endothelial cells (Hallmann, 2005)
Insulin-producing ß-cells (Nikolova, 2006)


Biolaminin 332 LN (LN332)

Keratinocytes (Ghohestani, 2001)




Biolaminin 221 LN (LN221)

Skeletal muscle (Patton, 1999)
Heart muscle tissue (Ja, 2016)


Biolaminin 211 LN (LN211)

Skeletal muscle (Patton, 1999)
Smooth muscle (Relan, 1999)
Certain neurons (Lathia, 2007)
Heart muscle tissue (Ja, 2016)


Biolaminin 121 LN (LN121)

ES/iPS differentiation (Miner, 2004)
Neurite outgrowth (Sasaki, 2010)


Biolaminin 111 LN (LN111)

ES/iPS differentiation (Miner, 2004)


1. Slowly thaw recombinant laminins at +4°C before use. 

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes, and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spread evenly across the surface. Note that the laminin matrix will be inactivated if let dry.


  • The laminin stock solution is long-term stable when stored at -20°C to -80°C. Please refer to the product-specific CoA for shelf life details.

  • Repeated freeze-thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. Thawed, undiluted laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions.

  • Avoid long exposure of the protein to ambient temperatures.

  • For your convenience, the coated plates can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

  • The protocol can easily be made totally defined and animal origin-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

Product name



Product code




For research use only





-20°C to -80°C


Stock concentration

4 x 0.1mg/ml



Clear, colorless, buffered solution with a 
pH of 7.2 with 10% glycerol and 0.02% NaN3


Shipping Condition

Dry Ice

Protein name

Laminin 521, 511, 421, 411, 332, 221, 211, 121 and 111



Defined and animal origin-free, human recombinant proteins 


Product application

Cell culture substrate. Laminins are expressed tissue-specifically in basement membranes of the extracellular matrix lining all epithelia-endothelia and covering other cell/organ types such as adipose tissue and muscle fibers.