LAMscreen

LAMscreen™


Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific. 

LAMscreen allows you to test which laminins that are optimal for your in vitro cell culture.

LAMscreen is a kit that contains all our currently available laminin isoforms in smaller quantities
(20 ug) which provides an easy and efficient way for determining the right laminin for your cells.

 

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Amount
Size
Price
Oty
140µg 8 x 20 ug €85
For larger quantities contact us

Estimated shipping time
1-5 working days

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Robust cell culture is achieved by cultuing cells on their natural cell adhesion molecule, laminin. All cells in the body make close contact with the extracellular matrix and one component, the basement membrane, surrounds all organs and functional units in vivo. The basement membrane is important for cell anchorage, survival, migration, differentiation and function.

There are many proteins in the basement membrane, but laminins are tissue-specific and play major roles in creating and sustaining cellular niches.

To date, more than 16 different laminin isoforms have been described. Through studies of human diseases and gene deletion experiments, the functions of a few isoforms have been elucidated. However, the exact role for most isoforms as well as the composition of laminins in different cell niches is still unknown.

In order to maintain proper function of cells and to differentiate them towards desired cell phenotypes, correct replication of the laminin foundation in the in vivo cell niche is essential.

LAMscreen makes it easy for you to test which laminin(s) provides the best support for your cells in vitro.

The following laminin isoforms have been demonstrated to serve critical roles for the listed cell types:

 

Laminin-521

Single-cell human ES/iPS cell culture (Rodin, 2014)
Clonal pluripotent stem cell culture (Rodin, 2014)
Podocytes (Miner, 2011)

 

Laminin-511

Culture of human ES/iPS cells (Rodin, 2010)
RPE-cells culture (Aisenbrey, 2006)
Hair follicles (Li, 2003)
Certain neuronal cells (Fusaoka-Nishioka, 2011)

 

Laminin-421

Mesangial cell culture (Abrass, 2010)
Kidney microvasculature (Thyboll, 2002)
Motorneuron synaptic transmisson (Patton, 2001)

 

Laminin-411

Endothelial cells (Hallmann, 2005)
Insulin producing ß-cells (Nikolova, 2006)

 

 

  

Laminin-221

Skeletal muscle (Patton, 1999)
Heart muscle tissue (Ja, 2016)

 

Laminin-211

Skeletal muscle (Patton, 1999)
Smooth muscle muscle (Relan, 1999)
Certain neurons (Lathia, 2007)
Heart muscle tissue (Ja, 2016)

 

Laminin-121*

ES/iPS differentiation (Miner, 2004)
Neurite outgrowth (Sasaki, 2010)

 

Laminin-111

ES/iPS differentiation (Miner, 2004)

 

*is currently supplied as a free sample

COATING PROCEDURE

1. Slowly thaw recombinant laminins at +4°C before use. Thawed laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions. For your convenience, the coated plates and diluted coating solution can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spreaded evenly across the surface. Note that the laminin matrix will be inactivated if let dry.

IMPORTANT NOTES

  • The Laminin stock solution is stable for 2 years when stored at -20°C. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C. Repeated freeze thawing should be avoided. 

  • The protocol can easily be made totally defined and xeno-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

  • Coat the wells in advance with the laminin solution according to the instruction here

Product name

LAMscreen™

 

Product use

Cell culture

 

Product application

Cell culture substrate 


 

Documents

MSDS

Storage

-20°C

 

Stability

Two years from date of receipt

 

Stock concentration

0.1mg/ml

 

Use concentration

0.5-2 ug/cm2
Optimal concentration must be determined by the end user

Protein name

Laminin-521, laminin-511, laminin-421, laminin-411, laminin-221, laminin-121, laminin-211 and laminin-111

 

Synonyms

Laminin-11, laminin-10, laminin-9, laminin-8, laminin-4, laminin-3, laminin-2 and laminin-1

 

Expressed in vivo

Laminins are expressed tissue-specifically in basement membranes of the extracellular matrix lining all epithelia-endothelia and covering other cell/organ types such as adipose tissue and muscle fibers.