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PRODUCT RECOMMENDATION

LAMININ-521LAMININ-511 and/or LAMININ-411


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CUSTOMER TESTIMONIALS          

HOW TO CULTURE MICROGLIAL CELLS ON LAMININ SUBSTRATES

Neural stem cell culture on laminin-521Microglial cells - the resident macrophages/immune cells in the central nervous system

Inflammatory responses to CNS damage are mediated by microglia. Activation of microglia is a complex, multistage process making them capable of releasing a large number of substances that can act both detrimental or beneficial for the surrounding cells. Recent studies indicate though that even in the normal brain, microglia have highly motile processes reaching out to synapses, eliminating unwanted connections in a process called pruning.

Microglia are of myeloid nature and ontogenetically related to macrophages, but their origin is still debated. During early fetal development, microglia derive from primitive myeloid macrophages in the yolk sac, seed the brain and under steady state conditions they will maintain themselves until adulthood. However, in response to CNS damage, numbers of microglia increase, which can be explained by the recruitment of monocytes from the bone marrow, supplementing the resident microglial population.

Laminin is a crucial factor to drive microglia into an inflamed state

In a recent study, Pietrogrande and colleagues have addressed the potential role of the extracellular matrix protein laminin as a crucial factor to drive microglia into an inflamed state (Pietrogrande, 2017). Chronic restraint stress of C57BL6 adult mice resulted in elevated levels of Laminin-α1 and pro-inflammatory markers such as TNF-α and iNOS. Immunolabeling of Laminin-α1 identified pyramidal neurons and dentate gyrus to be their primary source within the hippocampus. Furthermore, Iba-1 staining of microglia revealed that chronic stress also strongly reduced the total branch length, number of primary branches and number of branching points  when compared to microglia of control mice. In vitro, primary microglia and BV2 cells grown on Laminin-111 expressed higher levels of TNF-α, IL-1β and iNOS. In addition, LPS activation of microglia coated on Laminin-111 led to an increased proinflammatory state represented by higher pro-inflammatory cytokines level and phagocytic capability, both before and after stimulation. Interestingly, similar to observations made in vivo, microglia cultured on Laminin- 111 had fewer ramifications compared to control. These results expose the capability of chronic restraint stress in modulating Laminin within the CNS, an effect that has implications for understanding environmental mediated disturbances of microglial function.
 

Laminins relevant to microglia

Known is:

  1. The main laminin isoforms synthesized by human bone marrow stromal cells are laminin-411/421 and laminin-511/521 (Siler, 2000) 
  2. Microglia constitutively express a6b1 integrin (Milner and Campbell, 2002)
  3. Monocytes synthesize and migrate on laminin-411 and adhere to laminin-411 via a6b1 integrin (Pedraza, 2000)
  4. Laminin-511/521 and laminin-411 are adhesive proteins for precursors of myelomonocytic cell lineages (Gu, 2003)

 

LAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on laminin-521

  • Microglia constitutively express a6b1 integrin, a common receptor of laminin-521

  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment for human ES and iPS cell expansion and differentiation

  • No lot-to-lot variability for standardized experiments with less variation

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.