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BIOLAMININ 521BIOLAMININ 511 and/or BIOLAMININ 411


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HOW TO CULTURE MICROGLIAL CELLS ON BIOLAMININ SUBSTRATES

Neural stem cell culture on laminin-521Microglial cells - the resident macrophages/immune cells in the central nervous system

Inflammatory responses to CNS damage are mediated by microglia. Activation of microglia is a complex, multistage process making them capable of releasing a large number of substances that can act both detrimental or beneficial for the surrounding cells. Recent studies indicate though that even in the normal brain, microglia have highly motile processes reaching out to synapses, eliminating unwanted connections in a process called pruning.

Microglia are of myeloid nature and ontogenetically related to macrophages, but their origin is still debated. During early fetal development, microglia derive from primitive myeloid macrophages in the yolk sac, seed the brain and under steady state conditions they will maintain themselves until adulthood. However, in response to CNS damage, numbers of microglia increase, which can be explained by the recruitment of monocytes from the bone marrow, supplementing the resident microglial population.

Laminin is a crucial factor to drive microglia into an inflamed state

In a recent study, Pietrogrande and colleagues have addressed the potential role of the extracellular matrix protein laminin as a crucial factor to drive microglia into an inflamed state (Pietrogrande, 2017). Chronic restraint stress of C57BL6 adult mice resulted in elevated levels of laminin-α1 and pro-inflammatory markers such as TNF-α and iNOS. Immunolabeling of laminin-α1 identified pyramidal neurons and dentate gyrus to be their primary source within the hippocampus. Furthermore, Iba-1 staining of microglia revealed that chronic stress also strongly reduced the total branch length, number of primary branches and number of branching points  when compared to microglia of control mice. In vitro, primary microglia and BV2 cells grown on Biolaminin 111 expressed higher levels of TNF-α, IL-1β and iNOS. In addition, LPS activation of microglia coated on Laminin-111 led to an increased proinflammatory state represented by higher pro-inflammatory cytokines level and phagocytic capability, both before and after stimulation. Interestingly, similar to observations made in vivo, microglia cultured on Biolaminin 111 had fewer ramifications compared to control. These results expose the capability of chronic restraint stress in modulating Laminin within the CNS, an effect that has implications for understanding environmental mediated disturbances of microglial function.
 

Laminins relevant to microglia

Known is:

  1. The main laminin isoforms synthesized by human bone marrow stromal cells are laminin 411/421 and laminin-511/521 (Siler, 2000) 
  2. Microglia constitutively express a6b1 integrin (Milner and Campbell, 2002)
  3. Monocytes synthesize and migrate on laminin 411 and adhere to laminin 411 via a6b1 integrin (Pedraza, 2000)
  4. Laminin isoforms 511, 521 and 411 are adhesive proteins for precursors of myelomonocytic cell lineages (Gu, 2003)

 

BIOLAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on Biolaminin 521

  • Microglia constitutively express a6b1 integrin, a common receptor of laminin 521

  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment for human ES and iPS cell expansion and differentiation

  • No lot-to-lot variability for standardized experiments with less variation

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG human stem cell matrix makes pluripotent stem cell culture easy in a defined, animal component-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Laminin 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN (LN521) human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.