PRODUCT RECOMMENDATION

LAMININ-511 and/or LAMININ-111


Buy human recombinant laminin-521 cell culture reagents at BioLamina                                             Go to the BioLamina shop

Do you want us to contact you? Please klick here


PROTOCOL

Breast tumor cell migration and invasion assay 


CUSTOMER TESTIMONIALS        

HOW TO CULTURE HUMAN MAMMARY CELLS ON LAMININ SUBSTRATES

Culture of normal and cancerous mammary gland cells on defined and xeno-free human recombinant laminin cell culture reagentsLaminin have both overlapping and unique functions in the mammary gland tissue

Mammary ducts consist of an epithelial layer with basal connection to a single layer of myoepithelial cells. The duct is encircled by a basement membrane sheet that is intimately connected with the myoepithelial cells (Fata, 2003). The mammary basement membrane mainly contains, laminin-111, laminin-332, laminin-511, laminin-521 and collagen IV. Changes in the basement membrane laminin composition influence stem cell maintenance and mammary development, but also plays a role during aging and in aging-related diseases, such as cancer (Hu, 2017; Fata 2003). The extracellular matrix not only helps to support mammary basic structure, but also serves as a communicating bridge between mammary epithelia and their local and global environment throughout development. The major integrin receptors in the mammary gland are α3β1, α6β1 and α6β4 and the interaction between mammary epithelial cells and their extracellular matrix is crucial in the development and function of the tissue, affecting cell survival, polarity, proliferation, differentiation, adhesion, and migration (Hu, 2017; Muschler, 2010; Fata, 2003).
 

Laminin-111 is important for the initiation of mammary gland development, for ductal elongation and end bud persistence

End buds are surrounded by a basement membrane which is rich in laminin-111 and collagen IV. Blocking laminin-111 or β1 integrins specifically and dramatically reduced both the number of end buds per gland and the extent of the mammary ductal network (Klinowska, 1999). Laminin-111 and laminin-332 are suggested to be involved in initiation of mammary gland development, where laminin-111 has been shown to be critical for ductal elongation and end bud persistence (Chia, 2007; Hu, 2017; Muschler, 2010). Laminin-111 is also important for mammary epithelial cells to form polarized acinar structures, express b-Casein and permit prolactin receptor signaling (Hu, 2017; Muschler, 2010).

Laminin-511 and laminin-521 control submandibular gland epithelial morphogenesis and is required for correct epithelial cell organization and lumen formation

Laminin-511 is abundant in the vasculature of normal human mammary glands (Chia, 2007). Published results show that laminin-511 is not essential for gland initiation, but plays an important role in initial cleft formation and epithelial morphogenesis. Laminin-511 I also necessary for sublingual gland formation and later in development, is required for epithelial cell organization and lumen formation (Rebustini, 2007; Hu, 2017; Muschler, 2010). In submandibular glands, laminin-511 and laminin-111 are present in epithelial clefts but during epithelial morphogenesis as branching begins, laminin-511 expression increases while laminin-111 decreases. The data suggests that submandibular gland epithelial morphogenesis is controlled by al α5-chain laminins (laminin-511 and laminin-521), regulated via β1 integrin signaling. Laminin-511 also regulates FGFR expression, independent of laminin-111, which reciprocally regulates the expression of the laminin α5 subunit (Rebustini et al., 2007).


Laminin-511 and laminin-521 promotes breast cancer cell migration and invasion

The laminin-511 and laminin-521 isoforms are expressed in high levels in breast tumors (Chia, 2007) and are suggested to play an autocrine role in mammary cancer progression (Chia, 2007). Laminin-111 and laminin-332 are both down-regulated in most advanced breast tumors, suggesting a tumor suppressing role (Muschler, 2010). Contrary, laminin-511 act as a functional substrate, regulating breast cancer metastasis. Laminin-511 is a potent adhesive substrate for both murine and human breast carcinoma cells and promotes strong haptotactic responses in metastatic lines, largely mediated by the α3 integrin (Chia, 2007; Kusuma, 2011). Chia et al. propose that laminin-511 contributes during the process of metastasis via the attachment of breast tumor cells to blood vessel walls. These results are in accordance with a publication by Chang et al. where the authors show that breast cancer stem cells produce alpha-5 laminin matrix that promotes self-renewal and tumor initiation via α6β1 integrin interaction. α5 chain laminins also activates the Hippo transducer TAZ which in turn regulates the transcription of the α5 laminin subunit, establishing a positive feedback loop that contributes to stemness in breast cancer (Chang, 2014). 

LAMININ KEY ADVANTAGES

  • The interaction between mammary epithelial cells and their extracellular matrix is crucial in the development and function of the tissue

  • The mammary basement membrane is rich in laminin-111, laminin-332, laminin-511 and laminin-521

  • Laminin-111 is important for the initiation of mammary gland development, critical for ductal elongation and end bud persistence

  • Laminin-511 plays an important role in initial cleft formation, is necessary for sublingual gland formation, and is required for epithelial cell organization and lumen formation

  • Laminin-511 is expressed in high levels in breast tumors and promotes breast cancer cell migration and invasion

  • Defined and xeno-free cell culture matrix for clinical compliance

  • No lot-to-lot variability for standardized experiments with less variation

  • Scientifically validated in high-impact journals

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.