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HOW TO CULTURE PHOTORECEPTORS ON BIOLAMININ SUBSTRATES

Photoreceptor culture on laminin cell culture substratesLaminin proteins constitute a vital part of the photoreceptor matrix

Adult photoreceptors have been shown to adhere selectively to recombinant laminin beta-2 chain (Hunter, 1992). Beta-2 chain laminin have also been suggested to play a role in directing photoreceptor development where its spatial and temporal expressed throughout embryonic development and adulthood (Libby, 1996).


Apart from presence in the retinal pigmented epithelium and neural neuroepithelium, the laminin beta-2 chain is also found in association with choroidal blood vessels and blood vessels in the neural retina. In both places, it is co-localized with laminin 111 and culture of human embryonic stem cells on laminin 111 has indeed shown to induce expression of photoreceptor markers (Gong, 2008). 

In a publication by Libby et al. they examine the expression of all known laminin chains within the retina. The interphotoreceptor matrix contains the laminin a3, a4, a5, b2, b3, g2, and g3 subunits, which suggests the presence of three laminins: laminin 332, 423 and 523. These isoforms were also expressed in the subretinal space during early development and may play an important role in photoreceptor development, production, stability and synaptic organization (Libby, 2000).


Based on the importance of the beta-2 chain and we recommend Biolaminin 521 for culture of photoreceptors. 

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Biolaminin 521 MX research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.