Science Room



Product recommendation

LN521

Adult photoreceptors have been shown to adhere selectively to recombinant laminin beta-2 chain (Hunter et al., 1992). Beta-2 chain laminin have also been suggested to play a role in directing photoreceptor development where its spatial and temporal expressed throughout embryonic development and adulthood (Libby et al., 1996).


Apart from presence in the retinal pigmented epithelium and neural neuroepithelium, the laminin beta-2 chain is also found in association with choroidal blood vessels and blood vessels in the neural retina. In both places, it is co-localized with laminin-111 and culture of human embryonic stem cells on laminin-111 has indeed shown to induce expression of photoreceptor markers (Gong et al., 2008). 

In a publication by Libby et al. they examine the expression of all known laminin chains within the retina. The interphotoreceptor matrix contains the laminin a3, a4, a5, b2, b3, g2, and g3 subunits, which suggests the presence of three laminins: laminin-332, laminin-423 and laminin-523. These isoforms were also expressed in the subretinal space during early development and may play an important role in photoreceptor development, production, stability and synaptic organization (Libby et al, 2000).


Based on the importance of the beta-2 chain and we recommend laminin-521 (LN521) for culture of photoreceptors. 

RECOMMENDED PUBLICATIONS

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.