Human recombinant laminin-411 |
| BioLamina Laminin 411 is an excellent solution, supporting many diverse tissue cell types, such as cells from the vascular, immune, nervous, hematopoietic and pancreatic systems, and can be key in keeping these cells in culture |
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Laminin-411 (laminin-8) is expressed in endothelial cells, adipocytes, lymphocytes and platelets, and muscle cells as well as pancreatic cells, salivary and gastric glands, and epidermal cells in the developing and adult human body (Geberhiwot et al., 2001; Gu et al., 2003; Petäjäniemi et al., 2002). |
Essential for microvessel growth |
Important for insulin production |
Homing, extravasation, and survival |
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Endothelial cells |
β cell islets |
Blood cells |
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Importance for microvessel growthOne of the main isoform of laminins in capillaries and larger vessels produced by endothelial cells is Laminin-411 and, thus, their role in endothelial basal laminae function must be pivotal (Kortesmaa et al., 2000; Qian et al., 2007; Wondimu et al., 2004). Transgenic mice with an α4-chain laminin deficiency exhibit severe disorganization of microvessel growth, leading to microcirculation abnormalities (Thyboll et al., 2002; Wang et al., 2006). In addition, during sprouting angiogenesis laminins are produced and deposited from the stalk cells to the tip cell filopodia, and control the diameter size of the vessel lumen (Jakobsson et al., 2008), further supporting the importance of the protein in endothelial structure and function. |
Essential for insulin productionDifferentiation and insulin expression in the embryonic pancreas of β cells is initiated by and dependent on specific signals from capillary endothelial cells (Lammert et al., 2001). The specific outside in signals derive from extracellular proteins such as Laminin-411 and Laminin-511, which aggregated β cells, islets, are unable to produce. Therefore, they express vascular endothelial growth factor to attract endothelial cells, that form the essential protein secreting vascular network within the islets (Lammert et al., 2003; Nikolova et al., 2006). Data presented by Nikolova (2006) and colleagues suggest that treating islets with these laminins prior to transplantation will help maintain insulin production until new capillaries are formed in transplanted islets and that Laminin-411 and Laminin-511 in cell culture potentially could mimic the biological environment in the pancreas. BioLamina's revolutionary new modular matrix system brings together the unique qualities of Laminin-411 and Laminin-511 to provide the solution for making pancreatic beta cells thrive! |
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Homing, extravasation, survivalLymphoid cells are derived from bone marrow stem cells and differentiate either in the thymus or in the bone marrow. Lymphocytes are fundamental for innate and adaptive immunity and patrol the body for foreign antigens that activates them. Laminin-411 is secreted by lymphocytes and supports the proliferation, adhesion and migration of lymphocytes (Geberhiwot et al., 2001). Laminin-411 is also involved in the survival, adhesion and migration of blood neutrophils (Wondimu et al., 2004), and work as an adhesive substratum for bone marrow stem and progenitor cells and promote progenitor cell migration in vitro (Gu et al., 2003). |
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