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BIOLAMININ 521 

 
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Culture of neural stem cells and neurons derived from iPS cells on Biolaminin 521

neural stem cells and neurons derived from iPS cells cultured on laminin-521

 


REFERENCES

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CUSTOMER TESTIMONIAL 

"We have discovered LN521 is far superior to all other alternative ECMs we have tried for hPSC culture, and have switched all our hiPSC and hESC lines to LN521 for maintenance. As a result our hPSC lines have become more stable, yields increased and survival improved dramatically on passage. We can now perform more experiments with each flask, and do so with greater confidence of high quality results due to the morphological uniformity of pluripotent cells grown on LN521. We’ve also found LN521 performs best for the adherence and regional specification of NPCs and neurons across several CNS differentiation protocols, including the dorsal cortex, medial ganglionic eminence, ventral midbrain and ventral hindbrain."

              Dr. Jonathan Niclis, University of Copenhagen, Denmark


More Biolaminin product testimonials here

HOW TO CULTURE NEURAL STEM CELLS ON BIOLAMININ SUBSTRATES

Neural stem cell culture on laminin-521Laminin expression in the adult brain

Laminin 421, laminin 221, laminin 511 and laminin 521 are the main laminin isoforms expressed in the mouse and human subventricular/subependymal zone and ventricular zone (SVZ/SEZ and VZ) as well as the dentate gyrus of the adult central nervous system (Fietz, 2012; Hall, 2008; Indyk, 2003). Within the adult stem cell niches, the laminins are produced by ependymal cells, transit amplifying precursors (type C cells), GFAP+ astrocytes and vascular fractones in contact with the stem cells (Shen, 2008; Chen, 2003).

 

Laminin expression during development

The interaction between integrins and laminins is essential for adhesion and thus the survival, maintenance, proliferation and fate decisions of the neural stem cells (NSCs). Neuroepithelial cells and radial glial cells express laminin 511, laminin 521 and laminin 111. Additionally, other different laminin chains may play significant developmentally important roles, such as laminin 421 and laminin 221, which are expressed differently between radial glial cells and SVZ NSCs (Domogatskaya, 2012; Loulier, 2009).

 

Biolaminins for better culture of neural stem cells

Early studies using purified placental laminins (mainly laminin 521, 511, 411 and 211) show an increase in the proliferation rate of human NSCs derived from fetal tissue and reduced number of apoptotic cells (Hall, 2008). Human recombinant Biolaminin 521 and Biolaminin 511 substrates also support neural crest cells.

Watch this lecture by Associate Professor Anna Falk where she presents her research on patient specific neural stem cells which faithfully mimics known neurological disorder phenotypes in vitro. Patient-derived iPSCs are specified to neuroepithelial stem cell lines, efficiently expanded and differentiated into neurons and glial cells on Biolaminin 521.

BIOLAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on LN521.

  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment for human ES and iPS cells 

  • No lot-to-lot variability for standardized experiments with less variation

  • Homogenous and genetically stable iPSC cultures without need to remove differentiated parts

  • Effective proliferation of human neural stem cells

  • LN521 triggers the authentic cell signaling pathways which generates hPSCs with a more uniform gene expression profile

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG human stem cell matrix makes pluripotent stem cell culture easy in a defined, animal component-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Laminin 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN (LN521) human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.