Science Room

PRODUCT RECOMMENDATION

LAMININ-521 

 
                                             Go to the BioLamina shop

Do you want us to contact you? Please klick here


VEIW ON DEMAND

Culture of neural stem cells and neurons derived from iPS cells on laminin-521

neural stem cells and neurons derived from iPS cells cultured on laminin-521

 


REFERENCES

Find a selection of related articles here

 


CUSTOMER TESTIMONIAL 

"We have discovered L521 is far superior to all other alternative ECMs we have tried for hPSC culture, and have switched all our hiPSC and hESC lines to L521 for maintenance. As a result our hPSC lines have become more stable, yields increased and survival improved dramatically on passage. We can now perform more experiments with each flask, and do so with greater confidence of high quality results due to the morphological uniformity of pluripotent cells grown on L521. We’ve also found L521 performs best for the adherence and regional specification of NPCs and neurons across several CNS differentiation protocols, including the dorsal cortex, medial ganglionic eminence, ventral midbrain and ventral hindbrain."

              Dr. Jonathan Niclis, University of Copenhagen, Denmark


More laminin product testimonials here

HOW TO CULTURE NEURAL STEM CELLS ON LAMININ SUBSTRATES

Neural stem cell culture on laminin-521Laminin expression in the adult brain

Laminin-421, laminin-221, laminin-511 and laminin-521 are the main laminin isoforms expressed in the mouse and human subventricular/subependymal zone and ventricular zone (SVZ/SEZ and VZ) as well as the dentate gyrus of the adult central nervous system (Fietz, 2012; Hall, 2008; Indyk, 2003). Within the adult stem cell niches, the laminins are produced by ependymal cells, transit amplifying precursors (type C cells), GFAP+ astrocytes and vascular fractones in contact with the stem cells (Shen, 2008; Chen, 2003).

 

Laminin expression during development

The interaction between integrins and laminins is essential for adhesion and thus the survival, maintenance, proliferation and fate decisions of the neural stem cells (NSCs). Neuroepithelial cells and radial glial cells express laminin-511, laminin-521 and laminin-111. Additionally, other different laminin chains may play significant developmentally important roles such as laminin-421 and laminin-221, which are expressed differently between radial glial cells and SVZ NSCs (Domogatskaya, 2012; Loulier, 2009).

 

Laminins for better culture of neural stem cells

Early studies using purified placental laminins (mainly laminin-521, -511, -411 and -211) show an increase in the proliferation rate of human NSCs derived from fetal tissue and reduced number of apoptotic cells (Hall, 2008). Laminin-521 and laminin-511 also support neural crest cells.

Several research groups are successfully using laminin-521 to culture neural stem cells. Watch this lecture by Associate Professor Anna Falk where she presents her research on patient specific neural stem cells which faithfully mimics known neurological disorder phenotypes in vitro. Patient-derived iPSCs are specified to neuroepithelial stem cell lines, efficiently expanded and differentiated into neurons and glial cells on laminin-521.

LAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on laminin-521.

  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment for human ES and iPS cells 

  • No lot-to-lot variability for standardized experiments with less variation

  • Homogenous and genetically stable iPSC cultures without need to remove differentiated parts

  • Effective proliferation of human neural stem cells

  • Laminin-521 triggers the authentic cell signaling pathways which generates hPSCs with a more uniform gene expression profile

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.