Science Room

PRODUCT RECOMMENDATION

Motor neurons: LAMININ-211
Sensory neurons: LAMININ-411,  LAMININ-511 or LAMININ-111
Neuromuscular junctions: LAMININ-421


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REFERENCES

Find a selection of related articles here 
 


CUSTOMER TESTIMONIALS          

HOW TO CULTURE SPINAL PNS NEURONS ON LAMININ SUBSTRATES

Neural stem cell culture on laminin-521Sensory Neurons

Sensory trigeminal ganglion neurons grow well on laminin-411, but not on laminin-211 (Fried, 2005). Adult dorsal root ganglion (DRG) neurons are differentially regulated by laminins where laminin-111 and laminin-511 show the highest stimulation of neurite outgrowth (Plantman, 2008). Laminin-332 suppresses the mechanotransduction and axonal branching of cultured DRG sensory neurons. (Chiang, 2011)

 

Motor Neurons

Motor neurons in culture extend longer neurites on laminin-211 compared to laminin-411 (Wallquist, 2005). Wallquist and colleagues also showed that loss of α4 (laminin-411 and laminin-421) leads to a disturbance in radial sorting and impaired myelination (Wallquist, 2005; Chand, 2017).

 

Neuromuscular Junctions

Laminin-211 is also present together with laminin-221, laminin-421, and laminin-521 at neuromuscular junctions and ensures correct localization of pre- and postsynaptic specializations (Sanes and Lichtman, 2001; Patton, 2001; Domogatskaya, 2012).

LAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on laminin-521.

  • Sensory trigeminal ganglion neurons grow well on laminin-411

  • Laminin-111 and laminin-511 show the highest stimulation of neurite outgrowth

  • Motor neurons in culture extend long neurites on laminin-211

  • Laminin-211 is also present together with laminin-221, laminin-421, and laminin-521 at neuromuscular junctions
  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment 

  • No lot-to-lot variability for standardized experiments with less variation

  • Effective proliferation of human neural stem cells

  • Laminin-521 triggers the authentic cell signaling pathways which generates hPSCs with a more uniform gene expression profile

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.