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PRODUCT RECOMMENDATION

Motor neurons: BIOLAMININ 211
Sensory neurons: BIOLAMININ 411,  BIOLAMININ 511 or BIOLAMININ 111
Neuromuscular junctions: BIOLAMININ 421


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REFERENCES

Find a selection of related articles here 
 


CUSTOMER TESTIMONIALS          

HOW TO CULTURE SPINAL PNS NEURONS ON BIOLAMININ SUBSTRATES

Neural stem cell culture on laminin-521Sensory Neurons

Sensory trigeminal ganglion neurons grow well on laminin 411, but not on laminin 211 (Fried, 2005). Adult dorsal root ganglion (DRG) neurons are differentially regulated by laminins where laminin isoforms 111 and 511 show the highest stimulation of neurite outgrowth (Plantman, 2008). Laminin 332 suppresses the mechanotransduction and axonal branching of cultured DRG sensory neurons. (Chiang, 2011)

 

Motor Neurons

Motor neurons in culture extend longer neurites on Biolaminin 211 compared to when cultured on Biolaminin 411 (Wallquist, 2005). Wallquist and colleagues also showed that loss of α4 (laminin isoforms 411 and 421) leads to a disturbance in radial sorting and impaired myelination (Wallquist, 2005; Chand, 2017).

 

Neuromuscular Junctions

Laminin 211 is also present together with laminin 221, 421 and 521 at neuromuscular junctions and ensures correct localization of pre- and postsynaptic specializations (Sanes and Lichtman, 2001; Patton, 2001; Domogatskaya, 2012).

BIOLAMININ KEY ADVANTAGES

  • Patient-derived iPSCs are specified to neuroepithelial stem cell lines and efficiently expanded and differentiated into neurons and glial cells on Bolaminin 521.

  • Sensory trigeminal ganglion neurons grow well on Biolaminin 411

  • Biolaminin 111 and Biolaminin 511 show the highest stimulation of neurite outgrowth

  • Motor neurons in culture extend long neurites on Biolaminin 211

  • Laminin 211 is also present together with laminin isoforms 221, 421, and 521 at neuromuscular junctions
  • Defined and xeno-free cell culture matrix for clinical compliance

  • A biologically relevant culture environment 

  • No lot-to-lot variability for standardized experiments with less variation

  • Effective proliferation of human neural stem cells

  • Biolaminin 521 triggers the authentic cell signaling pathways which generates hPSCs with a more uniform gene expression profile

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG human stem cell matrix makes pluripotent stem cell culture easy in a defined, animal component-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Laminin 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN (LN521) human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.