Bergström R, Ström S, Holm F, Feki A, Hovatta O.
Methods Mol Biol. 2011;767:125-36.
A book chapter describing four different animal-protein free culture systems that are proven efficient in expanding pluripotent human ES cell populations. Two of the systems are with laminin-511 as substrate but with different medium, and the other two substrates are CELLstart and human foreskin fibroblasts.
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Hongisto H, Vuoristo S, Mikhailova A, Suuronen R, Virtanen I, Otonkoski T, Skottman H.
Stem Cell Res. 2012 Jan;8(1):97-108.
The authors of this study compared hESC supportive capacity of human fibroblast feeders from either dermis or foreskin together with a culture medium based on human serum. They found that only the fibroblasts from foreskin supported hESC self-renewal in this setting. Interestingly, when they compared protein expression levels between the different feeders, only foreskin fibroblasts synthesized laminin-511 as well as other proteins important for maintenance of hESC pluripotency.
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Rodin S, Domogatskaya A, Ström S, Hansson EM, Chien KR, Inzunza J, Hovatta O, Tryggvason K.
Nat Biotechnol. 2010 Jun;28(6):611-5.
In this article the authors describe, for the first time, the use of Laminin-511 as a substrate for human ES and iPS cells in vitro. The culture system is defined and devoid of animal products and feeder cells. Human pluripotent cells cultured on LN-511 substrate in this way maintained self-renewal capacity and pluripotency long-term, as well as karyotypic stability. Human ES cells plated on laminin-511 grow as a monolayer, which makes cell homogeneity particularly high.
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Azarin SM, Palecek SP.
Cell Stem Cell. 2010 Jul 2;7(1):7-8.
A commentary of three papers published almost simultaneously that describe defined, animal-protein free substrates for propagation of pluripotent human ES cells. One of those is laminin-511, and the other are a peptide-acrylate surface and a methacrylate-based polymer.
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Domogatskaya A, Rodin S, Boutaud A, Tryggvason K.
Stem Cells. 2008 Nov;26(11):2800-9.
Different laminin isoforms, LN-511, -332, -411 and -111, and Matrigel, gelatin and poly-D-lysine are compared as substrata maintaining pluripotent mouse ES cells in vitro without addition of leukemia inhibitory factor. Conclusions are that only LN-511 is able to sustain self-renewal for up to 169 days of culturing and cells maintain expression of pluripotency markers and can be used for generation of chimeric mice.
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Wondimu Z, Gorfu G, Kawataki T, Smirnov S, Yurchenco P, Tryggvason K, Patarroyo M.Matrix Biol. 2006 Mar;25(2):89-93.
Scientific paper which characterizes and compares commercial laminins isolated from human placenta to mouse laminin-111 and human recombinant laminin-211, -411, and -511 by ELISA, silver staining and Western blotting. One of the major finding is that laminin preparations from human placenta consisted of fragmented proteins, different laminin isoforms and unwanted, contaminating fibronectin. Due to the undefined and heterogenous nature of these preparations, the difference between batches was very pronounced.
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