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LN521

Endothelial Cells

Endothelial cells making up the vascular network in all organs of the body express and secrete laminin-411 and laminin-511 (Hallman et al., 2005). During angiogenesis laminins are produced and deposited from the stalk cells to the tip cell filopodia (Jakobsson, 2008) and laminin-521 was recently shown to be crucial for retinal angiogenesis (Gnanaguru et al., 2013).

 

Laminin-521 can be used as an optimal matrix for primary endothelial cells

Mature endothelial cells are often grown on fibronectin and this result in a de-differentiation of cells within hours to days that can be seen by complete loss of specific endothelial markers such as the von Willenbrandt factor (vWF). However, if instead growing primary endothelial cells, such as human umbilical cord endothelial cells (HUVECs), on laminin-521 or laminin-511 or laminin-411 we have successfully been able to culture HUVECs for as long as 20 passages with continuously strong expression of vWF (unpublished data).

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Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

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