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LN521

Endothelial Cells

Endothelial cells making up the vascular network in all organs of the body express and secrete laminin-411 and laminin-511 (Hallman et al., 2005). During angiogenesis laminins are produced and deposited from the stalk cells to the tip cell filopodia (Jakobsson, 2008) and laminin-521 was recently shown to be crucial for retinal angiogenesis (Gnanaguru et al., 2013).

 

Laminin-521 can be used as an optimal matrix for primary endothelial cells

Mature endothelial cells are often grown on fibronectin and this result in a de-differentiation of cells within hours to days that can be seen by complete loss of specific endothelial markers such as the von Willenbrandt factor (vWF). However, if instead growing primary endothelial cells, such as human umbilical cord endothelial cells (HUVECs), on laminin-521 or laminin-511 or laminin-411 we have successfully been able to culture HUVECs for as long as 20 passages with continuously strong expression of vWF (unpublished data).

Recommended publications

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Mesenchymal stem cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.