BioLamina's Scientific Advisor, Dr. Stephen Minger, talks about the development and current needs related to cell culture substrates.

 

 

Tissue specific laminins

Laminins are tissue-specific glycoproteins in the basement membrane, important for cell proliferation, migration and differentiation, and tissue development.

Laminin-521 and laminin-511 are expressed by cells in the inner cell mass of the blastocyst and are therefore optimal as substrates for human pluripotent stem cells. Human recombinant laminin isoforms are developed for defined and xeno-free stem cell culturing and primary cell cultures.

Culture ES and iPS cells

Stem cell culturing of human embryonic stem cells and induced pluripotent stem cells on recombinant laminin matrices is easy and efficient. Here we show human ES cells, split with trypsin and plated as single cells on top of recombinant laminin-521 that grow 30 minutes after plating to 5 days when they are ready to be split again. During the first 1-2 days a picture was taken every 15 minutes. Days 3-5 a picture was taken every hour.

Human ES and iPS cells grow as monolayers in a completely defined and xeno-free cell culture environment that enables self-renewal and pluripotency without the use of ROCK inhibitors.

Stem cell culturing

Stem cell culturing is easy on human recombinant Laminin-521 (LN521). It facilitates self-renewal of both pluripotent human embryonic stem cells and induced pluripotent stem cells in a chemically defined, feeder-free and animal-protein free cell culture system.

LN521 allows the survival and expansion of human ES and iPS cells after plating from single cell suspension. LN521 based stem cell cultures grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation. To culture embryonic stem cells on LN521 enables easy and efficient expansion of your hESCs and iPSCs and provides a biorelevant in vitro stem cell niche.

Derivation and culture of stem cells and primary cells on specific laminins

Professor Karl Tryggvason discusses the use of biologically relevant human recombinant laminins from BioLamina that successfully recreates specific cell niches in the cell culture dish. This enables robust stem cell culture methods in highly physiological microenvironments that in practical solves all current technical challenges with culturing human ES and iPS cells. It also describes novel data for culturing primary cells such as cardiomyocytes, pancreatic beta cells and endothelial cells.

Biorelevant laminins

Dr. Kristian Tryggvason tells the story about BioLamina and why biologically relevant laminins are the best matrix for cell culturing, including culture of stem cells and primary cells.