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Nature Protocols

Clonal expansion of hPSCs with LN-521
STEP-BY-STEP guide

WEEKEND-FREE CULTURE

OF HUMAN PLURIPOTENT STEM CELLS ON LN-521™

The Science Room

Welcome to BioLamina's newly established Science Room!

  • x
  • Human pluripotent stem cell culture

  • Clonal pluripotent stem cell culture

  • RPE and photoreceptor cell culture

  • Culture beating cardiomyocytes

  • Cell culture of neural cell lineages

  • Mouse pluripotent stem cell culture

  • Maintaining endothelial cells in culture

  • Pancreatic cells and islets

  • Epithelium and keratinocyte culture

Laminin-521 Stem Cell Matrix

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Cell culture of neural cell lineages

  • Human pluripotent stem cell culture

  • Clonal pluripotent stem cell culture

  • RPE and photoreceptor cell culture

  • Culture beating cardiomyocytes

  • Mouse pluripotent stem cell culture

  • Maintaining endothelial cells in culture

  • Pancreatic cells and islets

  • Epithelium and keratinocyte culture

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Cell culture of neural cell lineages

  • Clonal pluripotent stem cell culture

  • RPE and photoreceptor cell culture

  • Human pluripotent stem cell culture

  • Maintaining endothelial cells in culture

  • Mouse pluripotent stem cell culture

  • Pancreatic cells and islets

  • Epithelium and keratinocyte culture

  • Culture beating cardiomyocytes

LAMscreen™

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.

 

 

“By using the nature of the laminins in vitro it is possible to create a biorelevant cell culture niche that ensures in vivo-like phenotypes for better experiments and clinical translation – a true revolution.”

- Dr. Kristian Tryggvason, CEO

 

The BioLamina Laminin Technology

BioLamina offers cell culture matrices mimicking natures own, providing the only laminins that are the original, full length, recombinant laminins on the market - making cell culture easy, reliable and repeatable

 

BioLamina's Latest

We at BioLamina are proud to introduce our new web page. Human recombinant Laminins for sale are found in our Shop, while The Science Room contains New Publications, Testimonials and lots of data. Recommendations on which laminins to use for particular cells can be found under Applications for Cell Culture. We are updating the web with new data weekly, so make sure you visit us regularly!

 

Events & Meetings

BioLamina will be attending World Stem Cell Summit 2014 in San Antonio, Texas December 3 - 5. Make sure to join us!


Poster presentations
ISSCR 2014 
ISCT 2014

Overview Events & Meetings 2014

 

News feed

To keep up to date with news from BioLamina, make sure you follow our updates on LinkedIn, Facebook and Twitter.

Testimonials

"We have changed our hESC and hiPSC cultures from a MEF-based system to Laminin-521 in combination with Nutristem medium. This system allows a more standardized culture, independent of variable batches of MEFs. We experienced that the laminin-521 culture system has a high efficiency and lower labor-intensity when compared to the MEF-based systems. LN521 enabled us to culture our cell lines more flexibly, using only small quantities of cells for routine maintenance of lines and allowing a fast upscaling of cultures when experiments are planned."
Doctor Mieke Geens, Medical School of the Vrije Universiteit

 

Want to hear what other researchers think of BioLamina's products? Take a look here.

BioLamina is proud to initiate interviews of people behind the important stem cell work being done across the globe. We are kicking this off with an interview with Associate Professor Anna Falk at Karolinska Institutet here in Stockholm, Sweden. Enjoy!

 

BioLamina Presents

Dr Anna Falk

In 2012 Anna Falk became an associate professor at Karolinska Institutet in Stockholm. She heads a research team studying molecular mechanisms underlying developmental diseases of the human nervous system. Using iPSC (induced Pluripotent Stem Cell) techniques she models the neural development of a human brain in a dish. Anna has also been tasked with establishing the first iPSCs core facility in Sweden and is in the middle of getting everything up and running. Read the interview.

Welcome to BioLamina's newly established laminin Science Room! Here you can read about the science behind the laminin technology, the evolution of stem cell culture, and the revolution of BioLamina's laminins. 

BioLaminas's products are based on decades of groundbreaking discoveries and are used by leading research groups world wide. We want to share this knowledge with you, and by launching The Science Room we have taken the first step in developing a platform for technical information related to laminin-based cell culture.

Please let us know how we can make The Science Room the forum of your choice.

 

 

Biolamina is in the forefront of cell culturing and offers premium high technology products combined with outstanding scientific support. Our products empower scientists to start a new era in medical research, ultimately enabling cell therapy.

We are known and recognized for our products, competence, dedication and service, all contributing to our customers’ efficiency and scientific breakthroughs.

Stem cell culture

Pluripotent stem cell culture is easy on human recombinant Laminin-521 (LN-521™). Laminin-521 is used as the stem cell substrate for self-renewal of both human embryonic stem cells and induced pluripotent stem cells in a chemically defined, feeder-free and animal-protein free cell culture system.

Laminin-521 allows the survival and expansion of human ES and iPS cells after plating from single cell suspension. LN-521™ based stem cell cultures grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation. Laminin-521 enables easy and efficient expansion of your hESCs and iPSCs in a biologically relevant in vitro stem cell niche.