CELL CULTURE ON BIOLOGICALLY RELEVANT LAMININ
Almost all cells in the human body grow on specific laminins. With our human recombinant laminin substrates, Biolaminins, pluripotent stem cells, adult stem cells and tissue-specific cells can now be cultured in a physiologically relevant environment. Cell culture of primary cells and stem cells is reliable and robust when growing your cells on the natural human recombinant laminin that match your cell of interest.
Read more under each cell type in science room and which laminin to order for your cell culture.
Animal stem cells
Cell culture of mouse pluripotent stem cells on Biolaminin 511 increases cell adhesion and cell proliferation. Cells can be grown without feeders and lif in a defined environment with less variance and improved quality of cell culture data.
Different cancer cells interact with laminin to activate signaling pathways promoting tumor cell growth, invasion and metastasis. Biolaminin 521 can also provide support for culture of various cancer cells, such as neuroblastoma and glioblastoma cells.
Chemically defined generation of human cardiomyocytes is improved with cardiac laminins that increase the number and adhesion of cardiomyocytes. Isolate, expand and differentiate primary cardiac progenitors and hPSCs with muscle laminins.
Clonal cell culture applications
Use the Biolaminin 521 matrix and protocol to increase survival of single hES and iPS at clonal densities for genome editing and single-cell sorting.
Endothelial cells and progenitors in culture can be maintained on endothelial-specific laminins for months with strong expression of endothelial marker vWF compared to fibronectin that leads to de-differentiation of endothelial cells within days.
Biologically relevant recombinant laminin, Biolaminin 511, provide a chemically defined and fully human platform for human epidermal keratinocyte culture.
Expansion of human PSC
Derivation, reprogramming, expansion and differentiation of human ES and iPS cells in completely chemically defined, feeder-free and animal component-free conditions is robust on Biolaminin 521 expressed by hES and iPS cells.
Culture retinal pigmented epithelial (RPE) cells, photoreceptors and other retinal cells on the laminins expressed in Bruch´s membrane and the neuroepithelium. Biolaminin 521 improve RPE cell culture.
Hematopoietic stem and progenitor cells
Expansion and maintenance of HSC and clinically compliant hematopoietic differentiation to megakaryocytes, platelets and red blood cells on the chemically defined and animal component-free Biolaminin 521 cell culture substrate.
Culture of human ES cells on human recombinant Biolaminin 521 and Biolaminin 111 substrates significantly improve hepatocyte differentiation, maturation, function and stabilization of phenotype.
Laminin has a vital role in the early developing intestine are crucial for establishing and maintaining the intestinal architecture.
Laminin-521 is expressed in kidney glomerular basement membrane and on podocytes where the laminin-integrin binding is essential for the typical glomerular capillary loop structure. Mesangial cells produce Laminin-421.
Laminins are crucial for lung development and are key proteins in the adult basement membranes of the airway and alveolar epithelium, endothelium, bronchial and vascular smooth muscle cells, nerve cells, and visceral pleura.
Mesenchymal stem cells
Laminin 521 is widely expressed in bone marrow. Biolaminin 521 is an efficient substrate for MSC adhesion and expansion.
Cell culture of dopaminergic neurons, neural stem cells, motor neurons and other nerve cells on their respective laminins increase adhesion, neurite outgrowth, maturity and functionality. Read about recommended laminins in the science room.
Normal and cancerous mammary cells
The mammary basement membrane is rich in laminin which largely influence stem cell maintenance and mammary development, but also cancer progression.
Expansion of primary pancreatic islets on pancreas-specific laminin 521 results in proliferation of beta, delta and alpha cells and endothelial cells. Laminin-411 increase differentiation to insulin producing beta cells from mesenchymal stem cells.
Skeletal muscle cells
Biolaminin 521 maintains the differentiation potential of human satellite cell-derived myoblasts, even after long-term expansion, support increased proliferation, superior differentiation, and myotubes with higher amounts of nuclei per myotube.