CELL CULTURE ON BIOLOGICALLY RELEVANT LAMININ
Cell culture of primary cells and stem cells is reliable and robust when growing your cells on the natural human recombinant laminin that match your cell of interest. Almost all cells grow on specific laminins in the human body and as these laminins are now available as recombinant laminins this makes it possible to culture cells in a physiologically relevant environment.
BioLamina offers defined cell culture environments that enable cell culture of pluripotent stem cells, adult stem cells and tissue-specific cells. Cell culture of motor neurons and dopaminergic neurons, neural stem cells, cardiomyocytes, endothelial cells, pancreatic beta cells and many more cell types on their biologically relevant laminin is now possible. Quality and reliability is increased with recombinant laminins.
Read more under each cell type in science room and which laminin to order for your cell culture.
Animal stem cells
Cell culture of mouse pluripotent stem cells on laminin-511 increases cell adhesion and cell proliferation. Cells can be grown without feeders and lif in a defined environment with less variance and improved quality of cell culture data.
Different cancer cells can interact with laminin to activate signaling pathways promoting tumor cell growth, invasion and metastasis. Laminin-521 can also provide support for culture of various cancer cells, such as neuroblastoma and glioblastoma cells.
Chemically defined generation of human cardiomyocytes is improved with cardiac laminins that increase the number and adhesion of cardiomyocytes. Isolate, expand and differentiate primary cardiac progenitors and hPSCs with muscle laminins.
Clonal cell culture applications
Use the LN-521™-based matrix and protocol to increase survival of single hES and iPS at clonal densities for genome editing and single-cell sorting.
Endothelial cells and progenitors in culture can be maintained on endothelial-specific laminins for months with strong expression of endothelial marker vWF compared to fibronectin that leads to de-differentiation of endothelial cells within days.
Laminin-332 is enriched in epithelial basement membranes and influences proliferation of keratinocytes and is, thus, upregulated during wound healing and importantly affects keratinocyte migration, invasion and eventually skin tissue remodeling.
Expansion of human PSC
Derivation, reprogramming, expansion and differentiation of human embryonic stem cells and induced pluripotent stem cells in completely chemically defined, feeder-free and xeno-free conditions is robust on Laminin-521 expressed by hES and iPS cells.
Culture retinal pigmented epithelial (RPE) cells, photoreceptors and other retinal cells on the laminins expressed in Bruch´s membrane and the neuroepithelium. Laminin-521, laminin-511, laminin-332 and laminin-111 improve RPE cell culture.
Hematopoietic stem and progenitor cells
Expansion and maintenance of HSC and clinically compliant hematopoietic differentiation to megakaryocytes, platelets and red blood cells on the chemically defined and xeno-free laminin-521 cell culture substrate.
The liver contains several different laminin isoforms and experiments show that several laminins promote attachment of mature hepatocytes in culture flasks. For hepatoblasts, liver progenitors, Laminin-111 is an optimal laminin matrix.
Laminin has a vital role in the early developing intestine are crucial for establishing and maintaining the intestinal architecture.
Laminin-521 is expressed in kidney glomerular basement membrane and on podocytes where the laminin-integrin binding is essential for the typical glomerular capillary loop structure. Mesangial cells produce Laminin-421.
Laminins are crucial for lung development and are key proteins in the adult basement membranes of the airway and alveolar epithelium, endothelium, bronchial and vascular smooth muscle cells, nerve cells, and visceral pleura.
Mesenchymal stem cells
Laminin-521 is widely expressed in bone marrow and is an efficient substrate for MSC adhesion and expansion
Cell culture of dopaminergic neurons, neural stem cells, motor neurons and other nerve cells on their respective laminins increase adhesion, neurite outgrowth, maturity and functionality. Read about recommended laminins in the science room.
Normal and cancerous mammary cells
The mammary basement membrane is rich in laminin which largely influence stem cell maintenance and mammary development, but also cancer progression.
Expansion of primary pancreatic islets on pancreas-specific laminins results in proliferation of beta cells, delta cells, alpha cells and endothelial cells. Laminin-411 increase differentiation to insulin producing beta cells from mesenchymal stem cells.
Skeletal muscle cells
Laminin-211 is the most abundant laminin isoform in the basement membrane of adult skeletal muscle and mutations in the gene encoding laminin a2 chain (LAMA2) are the most common cause of congenital muscular dystrophy.