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LAMININ-521LAMININ-511 and LAMININ-332


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CUSTOMER TESTIMONIAL  

HOW TO CULTURE HAIR FOLLICLE STEM CELLS ON LAMININ SUBSTRATES

Hair follicle cell culture applications on human recombinant laminin substratesEpithelial tissues contain a-5 and a-3 laminin isoforms

Epithelial tissues are sheet of cells that line the surfaces and cavities of the whole body, including the skin, oral cavity, lung, gastrointestinal and urinary tracts, hair follicles and many glands. The epithelial cells are polarized with the basolateral surface resting on a basement membrane (BM) that contain high levels of alpha-5 laminins and laminin-332. Epithelial BM also contain laminin-311 and to a lesser extent laminin-321 (Domogatskaya, 2012). Laminin-311 forms a covalent complex with laminin-332 in epithelial tissue (Champliaud, 1996) and has been shown to participate in mechanically induced signal transduction after mechanical stimulation (Jones, 2005).


Laminin-332 and laminin-511 constitutes the hair follicle stem cell niche

Laminin-332 and laminin-511 is the major constituents of the BM of the hair follicle (Morgner, 2015). Laminin-332 expression is higher in the interfollicular epidermis (IFE) compared with the deeper portions of the hair follicle. Laminin-511 is less abundant beneath the IFE but with strongest expression at the isthmus region and around the hair germ (Morgner, 2015), essential for hair follicle down growth (Gao, 2008). Dermal papilla from laminin-511 mutants show developmental defects (Gao, 2008). Laminin-511 is the primary laminin of the early developing hair. Absence of laminin-511 in transgenic mice leads to a number of developmental abnormalities, including arrest of hair development and deficient Shh expression in hair follicles (Li, 2003). Skin grafts from Lama5-null mouse embryos grafted onto healthy mice fail to grow hair whereas purified laminin-511 restores hair development in the Lama5-null skin (Li, 2003). In human and in mice, absence of laminin-332 results in extensive skin blistering and lethality few days after birth, highlighting its importance for epidermal architecture (Morgner, 2015).


The ratio between laminin-332 and -511 regulate hair follicle bulge stem cells activation

The hair growth cycle involves three stages: the growth phase (anagen), the regression phase (catagen) and the quiescent phase (telogen). During each anagen phase, follicles is dividing rapidly, adding to the hair shaft. During the catagen and telogen phase, follicles reset and prepare their stem cells so that they can receive signal to start the next growth phase. Hair loss occurs when the balance between those stages changes. Laminin-511 is upregulated in the anagen phase and exogenous laminin-521 and laminin-511 makes adult human hair follicles grow faster (Sugawara, 2007). Laminin-511 is downregulated during anagen-to-catagen transition, and this downregulation is required for catagen progression. In contrast, Laminin-332 is upregulated in catagen and antagonized hair growth induced by laminin-511 (Sugawara, 2007). The integrin receptor subunit β1 is central for maintenance of the quiescent bulge stem cell pool (Morgner, 2015). Inhibition of the β1 integrin disrupted dermal papilla primary cilia formation and hair development (DeRouen, 2010).

Laminin-511 promotes Tgf-β signaling which makes hair follicle stem cells (HFSC) more receptive for activating signals. Contrary, laminin-332 suppresses Wnt/β-catenin signaling that is required for HFSC differentiation. The ratio between the two laminin isoforms regulate key signaling pathways that determine somatic stem cells activation within the bulge niche (Morgner, 2015).

Exogenous laminin-511 enhances the growth of hair follicle stem cells, whereas addition of laminin-332 antagonizes this effect. FACS-purified hair follicle stem cells attach very well to laminin-511 coated plates with about 6 times higher well area coverage compared to other cell culture matrices, such as collagen I, fibronectin or Matrigel (Lay, 2016).

LAMININ KEY ADVANTAGES

  • Defined and animal component-free cell culture matrix for clinical compliance.

  • A biologically relevant culture environment for epithelial cells

  • Laminin-332 and laminin-511 is the major constituents of the BM of the hair follicle.

  • Laminin-511 is the primary laminin of the early developing hair.

  • Laminin-511 is upregulated in the anagen phase and exogenous laminin-521 and laminin-511 makes adult human hair follicles grow faster.

  • Laminin-332 is upregulated in catagen and antagonized hair growth

  • The integrin receptor subunit β1 is central for maintenance of the quiescent bulge stem cell pool, a potent adhesive substrate for laminin-511 and laminin-521.

  • No lot-to-lot variability for standardized experiments with less variation.

  • Scientifically validated in high-impact journals.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Biolaminin 521 MX research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.