Laminin-111

LN111
Human recombinant laminin-111


Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific.

Human recombinant laminin-111 is important during early epithelial development and differentiation of the epiblast. Laminin-111 is up-regulated and widely expressed during embryogenesis, vital for the development of an embryo. However, its tissue distribution after birth is restricted to only a few tissues, such as brain and kidney. Laminin-111 is commonly used as a general attachment protein for most cell types in vitro and LN111 have been used successfully for hepatic and neural differentiation. 

BUY ONLINE!

Amount
Size
Price
Oty
100µg 1 x 1ml €69
1mg 10 x 1ml €610
For larger quantities contact us

Estimated shipping time
1-5 working days

SAVE MONEY

Buy larger quantities and save shipping cost.

SIZE GUIDE

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

FORMAT
CONC. (ug/cm2).
NO OF WELLS
AMOUNT (uq)

Laminin-111 supports the survival, proliferation and differentiation of many different cell types in vitro, although many of those cultivated cell types would not encounter the protein naturally in vivo. Instead you should match your specific cell type to the laminin naturally expressed by this cell which leads to improved cell culture.

During development Riechert’s membrane supporting the extraembryonic trophoblasts express laminin-111. Reichert’s membrane is essential for epiblast differentation, which is essential for the formation of the three embryonic germ layers by pluripotent cells in the inner cell mass.

In the adult, laminin α1 chain expression is relatively restricted and is only present in some epithelial basement membranes, and primarily found in tissues such as the eye, liver and kidney, whereas hardly expressed at all in endothelial, adipose, nervous, and muscle tissues (Ekblom, 2003; Virtanen, 2000).

It is unknown why laminin-111 can exert all these major biological effects on such a wide selection of cell types, but one explanation is that, depending on the cell type, laminin-111 can not only induce different cell signaling cascades but as all laminins also co-signal with various growth factors.

Laminin-111 is the laminin isoform present in the tumor extract sold under the trade name Matrigel. Matrigel is produced by mouse Engelbreth-Holm-Swarm (EHS) sarcoma cells and is an undefined mix of extracellular proteins of animal tumor origin.

However, by coating your plate with recombinant laminin-111 you support your cells with a defined and xeno-free substrate. Therefore, human recombinant laminin-111 enables better control of your cell matrix and experiments.

Laminin-111 is the main component in Matrigel that is used for stem cell culture. By using matrigel for human stem cells, laminin-111 initiates differentiation of stem cells instead of efficient self-renewal of stem cells. This can be seen as spontaneous differentiation of pluripotent stem cells in Matrigel or laminin-111 coated cell culture dishes.

For pluripotent stem cells, laminin-521 should instead be used for human stem cell culture and laminin-511 for mouse stem cell culture. This is because laminin-521 and laminin-511 are the natural laminins for hES and iPS cells and does not cause spontaneous differentiation. Instead they lead to efficient expansion and growth of pluripotent stem cells.

COATING PROCEDURE

1. Slowly thaw recombinant laminins at +4°C before use. Thawed laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions. For your convenience, the coated plates and diluted coating solution can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted Laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spreaded evenly across the surface. Note that the laminin matrix will be inactivated if let dry.

IMPORTANT NOTES

  • The Laminin stock solution is stable for 2 years when stored at -20°C. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C. Repeated freeze thawing should be avoided. 

  • The protocol can easily be made totally defined and xeno-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

  • Coat the wells in advance with the laminin solution according to the instruction here

Product name

LN111

 

Product use

Cell culture

 

Product application

General cell culture substrate


 

Documents

MSDS

Storage

-20°C

 

Stability

Three years from date of receipt

 

Stock concentration

0.1mg/ml

 

Use concentration

0.5-2 ug/cm2
Optimal concentration must be determined by the end user

Protein name

Laminin-111 (LN-111)

 

Synonyms

Laminin-1

 

Expressed in vivo

Expressed and secreted by trophectoderm and some epithelial basement membranes.