Biolaminin 211 LN

Biolaminin 211 LN (LN211)

Human recombinant laminin 211

Recreating the natural cell niche is key to successful cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue-specific. 

Human recombinant laminin 211 substrate, Biolaminin 211 LN (LN211), supports the growth, survival, and differentiation of a wide range of tissue-specific cell types, including motor neurons, cardiac cells, and skeletal muscle cells.



100 µg 1 x 1 ml €78
500 µg 1 x 5 ml €339
For larger quantities contact us

Estimated shipping time
1-5 working days

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Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

CONC. (ug/cm2).

Laminin 211 is crucial for muscle development and function and is one of the main isoforms present in adult muscle tissue, including varying amounts of laminin 221, laminin 521 and laminin 421 depending on the tissue.

Mutations of the LAMA2 gene are the most common cause of congenital muscular dystrophy that frequently leads to death in early childhood (Domogatskaya, 2012). For a review of laminin 211 in skeletal muscle function, see Holmberg and Durbeej, 2012.

Laminin 211 as well as laminin 221, are very important for cardiomyocytes and heart muscle development. In the developing heart both laminin 211 and laminin 221 are expressed in the extracellular matrix of cardiomyocytes as well as in the basement membrane zones of the endo- and pericardium and the capillaries (Roediger, 2010).

Laminin 211 is a major laminin isoform synthesized by Schwann cells in the developing peripheral nervous system, important for axonal elongation/neurite extension and myelinization (Patton, 2000; Patton, 2001; Wallquist, 2005).

Laminins containing the α2-chain (such as laminin 211) are expressed in the ventricular zone ECM in the developing mouse central nervous system. Since laminins are known to contribute to the stem cell niche in the brain, this suggests that laminin 211 could play an important functional role in the regulation of neural stem cells and progenitor cells (Haubst, 2006; Kazanis, 2010; Lathia, 2007; Mercier, 2002).

Authentic cardiomyocyte cell culture

Muscle cells

Cardiomyocytes, smooth and
skeletal muscle cell culture 


Authentic neuron cell culture laminin

Nervous system

CNS and PNS cell culture


1. Slowly thaw recombinant laminins at +4°C before use. 

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes, and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spread evenly across the surface. Note that the laminin matrix will be inactivated if let dry.


  • The laminin stock solution is long-term stable when stored at -20°C to -80°C. Please refer to the product-specific CoA for shelf life details.

  • Repeated freeze-thawing should be avoided. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C to -80°C. Thawed, undiluted laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions.

  • Avoid long exposure of the protein to ambient temperatures.

  • For your convenience, the coated plates can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

  • The protocol can easily be made totally defined and animal origin-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

Product name

Biolaminin 211 LN


Product code




For research use only






-20°C to -80°C


Stock concentration




Clear, colorless, buffered solution with a 
pH of 7.2 with 10% glycerol and 0.02% NaN3


Shipping Condition

Dry Ice

Protein name

Laminin 211 (Laminin-2)



Defined and animal origin-free, human 
recombinant protein 


Product application

Culture of skeletal and smooth muscle cells, cardiomyocytes, glial cells, and neural progenitor cells