Laminin-411

LN411
Human recombinant laminin-411


Recreating the natural cell niche is key to succesfull cultivation. The physical, topological, and biochemical expression of the different laminin isoforms in the BM is heterogeneous and tissue specific. 

Human recombinant laminin-411 supports many diverse tissue cell types such as cells from the pancreas and the vascular, immune, nervous, hematopoietic systems, and can be key when culturing these cells.

 

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Amount
Size
Price
Oty
100µg 1 x 1ml €69
1mg 10 x 1ml €610
For larger quantities contact us

Estimated shipping time
1-5 working days

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SIZE GUIDE

Not sure how much laminin you need? To make it easy, we have created a tool where you can calculate the amount needed for your experiments. Just choose culture well format and fill in the desired coating concentration to see the amount required.

FORMAT
CONC. (ug/cm2).
NO OF WELLS
AMOUNT (uq)

Recombinant human laminin-511 and laminin-411 can in combination create a natural niche in the culture dish for endocrine pancreatic beta cells, promoting both proliferation of β cells and insulin gene expression.

In the embryo, differentiation and insulin expression by the pancreas β cells is initiated by and dependent on specific signals from capillary endothelial cells (Lammert, 2001). However, pancreatic cells are unable to produce the extracellular proteins laminin-411 and laminin-511, which are expressed and secreted by endothelial cells.

Instead, the pancreatic islets express vascular endothelial growth factor to attract endothelial cells, providing both oxygen and nutrients and form the essential vascular network for insulin production by the β cells (Lammert, 2003; Nikolova, 2006).

Data presented by Nikolova (2006) and colleagues suggest that treating islets with these laminins prior to transplantation will help maintain insulin production until new capillaries are formed in transplanted islets and that laminin-411 and laminin-511 in cell culture potentially could mimic the biological environment in the pancreas.

BioLamina's revolutionary matrix system brings together the unique qualities of laminin-411 and laminin-511 to provide the solution for making pancreatic islet cells thrive.

 

DIFFERENTIATION AND MAINTENANCE OF PANCREATIC CELLS

One of the main isoform of laminins in capillaries and larger vessels produced by endothelial cells is laminin-411 and, thus, it has a pivotal role in endothelial basal laminae function (Kortesmaa, 2000; Qian, 2007; Wondimu, 2004). Transgenic mice with an α4-chain laminin deficiency exhibit severe disorganization of microvessels, leading to microcirculation abnormalities (Thyboll, 2002; Wang, 2006).

In addition, during sprouting angiogenesis laminins are produced and deposited from the stalk cells to the tip cell filopodia, and control the diameter size of the vessel lumen (Jakobsson, 2008), further supporting the importance of the protein in endothelial structure and function.

Lymphoid cells are derived from bone marrow stem cells and differentiate either in the thymus or in the bone marrow. Lymphocytes are fundamental for innate and adaptive immunity and patrol the body for foreign antigens that activates them. Laminin-411 is secreted by lymphocytes and supports the proliferation, adhesion and migration of lymphocytes (Geberhiwot, 2001). 

Laminin-411 is also involved in the survival, adhesion and migration of blood neutrophils (Wondimu, 2004), and works as an adhesive substratum for bone marrow stem and progenitor cells and promote progenitor cell migration in vitro (Gu, 2003).

Cell Culture of Beta Cells

Maintenance and expansion
of insulin producing islets 

 

Authentic endothelial cell culture

Endothelial cells

Maturation and maintenance
of blood vessels 

Laminins in the pancreas

 laminin ECM in pancreatic islets

Islet endothelial cells in mouse pancreas express both laminin-411 (left) and laminin-511 (right) seen as red in these confocal microscopy images. The laminins induce a cell-signaling cascade in the β islet cells, which leads to expression of insulin and cell proliferation.


 

Laminins by endothelial cells

laminin expression in pancreatic islets

Laminins and other extracellular proteins, including collagen IV, are only produced by islet endothelial cells and not by islet β cells. Laminin-411 and laminin-511 proteins act in concert with instructive and cell-type specific signals and are, in addition, required in liver morphogenesis and growth.


 

Laminins and insulin

Laminin maintain beta cell insulin expression culture

Laminin-411 and laminin-511 in cell culture can partially substitute the biological environment of the pancreas and can, therefore, potentially help maintain β cell insulin production during islet transplantations before the islets are revascularized by the body.


 

COATING PROCEDURE

1. Slowly thaw recombinant laminins at +4°C before use. Thawed laminin stock is stable for at least 3 months when stored at +2°C to +8°C under aseptic conditions. For your convenience, the coated plates and diluted coating solution can be kept for up to 4 weeks when stored aseptically at +2°C to +8°C.

2. Dilute the thawed laminin stock solution with 1xDPBS containingCa2+ and Mg2+.

3. Add the diluted laminin solution to tissue culture-treated cultureware for a final coating concentration of 0.5-2 ug/cm2. The optimal coating concentration is cell-dependent. See laminin coating instructions, recommended coating volumes and concentrations.

4. Seal the plate (e.g. with Parafilm®) to prevent evaporation and incubate at +2°C to +8°C overnight. If a more rapid coating is required, incubate at +37°C for 2 hours. Make sure the laminin solution is spreaded evenly across the surface. Note that the laminin matrix will be inactivated if let dry.

IMPORTANT NOTES

  • The laminin stock solution is stable for 2 years when stored at -20°C. If desired, the laminin stock can be dispensed into working aliquots and stored at -20°C. Repeated freeze thawing should be avoided. 

  • The protocol can easily be made totally defined and xeno-free with your choice of culture medium and enzyme.

  • Before start, all solutions used for cell passaging should be aliquoted in sufficient amounts and pre-warmed at +37°C, 5% CO2.

  • Coat the wells in advance with the laminin solution according to the instruction here

Product name

LN411

 

Product use

Cell culture

 

Product application

Pancreatic islet culture, endothelial cells, adipocytes and epidermal cells


 

Documents

MSDS

 

Storage

-20°C

 

Stability

Three years from date of receipt

 

Stock concentration

0.1mg/ml

 

Use concentration

0.5-2 ug/cm2
Optimal concentration must be determined by the end user

Protein name

Laminin-411 (LN-411)

 

Synonyms

Laminin-8

 

Expressed in vivo

Expressed and secreted by endothelial cells, adipocytes, lymphocytes and platelets, and muscle cells as well as pancreatic cells, salivary and gastric glands, and epidermal cells in the developing and adult human body.