Science Room

Cardiac Applications

PRODUCT RECOMMENDATION

LAMININ-521, LAMININ-211 and LAMININ-221


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PROTOCOL

Long-term expansion and differentiation of myogenic progenitors on laminin


CUSTOMER TESTIMONIALS

"Laminin-521 shifts away from the paradigm that muscle cells lose their ability to differentiate after culturing. Muscle differentiation on laminin-521 is nothing short of spectacular, yielding increased numbers of nuclei per myotube and improved myotube organization. Surprisingly, muscle cells expanded on laminin-521 maintain their ability to differentiate into myotubes after long-term passaging, better resembling differentiation of freshly isolated cells."

                                           Dr. Christopher Penton, Icagen, Inc., USA

         
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ARTICLES

HOW TO CULTURE CARDIAC CELLS ON LAMININ SUBSTRATES

HOW TO CULTURE CARDIAC CELLS ON LAMININ SUBSTRATESLaminin expression in muscle tissue

Laminin-211 and laminin-221 are expressed specifically in the basal lamina of striated muscles and an expression deficiency in the alpha-2 laminins results in muscular dystrophy, accompanied by a dilated cardiomyopathy (Oliviéro, 2000). Laminin isoforms containing the alpha-2, -4 and -5 subunits are critically important for the maintenance and development of heart muscle tissue (Miner, 1997). During stem cell cardiac differentiation, a distinct swish in expression profile of laminin subunits have been reported with laminin-411/421 pre-dominantly expressed early in progenitors and laminin-211/221 expressed later in cardiomyocytes (Ja, 2015). In the adult heart, the main laminin isoforms expressed are laminin-211 and laminin-221 but laminin-521 has also shown to be expressed.


Effective cardiomyocyte differentiation on laminin-211 and laminin-521

In a publication by a group of Japanese scientist, they have shown that with the use of cardiac muscle specific laminin-211, human iPSCs can be effectively be differentiated into cardiomyocytes using small molecules (Minami, 2012). The same researches later published another article with Shinya Yamanaka as co-author, where they again used laminin-211 for cardiac differentiation of human iPSC (Hirata, 2014). Laminin-211 has also been used for culture of adult human cardiomyocytes (Kuroda, 2015).


Laminin-521 also has properties that improves isolation and expansion of adult stem cells and progenitors. In a publication by Kuroda et al., iPSCs were efficiently expanded and differentiated to cardiomyocytes on laminin-521 (Kuroda, 2015). Dr. Joseph Wu´s lab also showed that laminin-521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes (Burridge, 2014). Laminin-521 was compared to 5 other feeder-free matrices and the authors concluded that:

  • Laminin-521 had the fastest human iPSC growth rate
  • Laminin-521 generated the highest number of cardiomyocytes
  • Only laminin-521 could sustain long-term adhesion (>15 d) during cardiac differentiation in chemically defined medium

 

This in in coordance with recent data presented by researchers at Icagen Inc. demonstrate that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). Laminin-521 support increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube. Moreover, Penton et al. show that laminin-521 support more consistent and reliable differentiation over long-term culture and is the only substrate facilitating high-level fusion following long-term culture. Laminin-521 support increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells and disease states. 

LAMININ KEY ADVANTAGES

  • Laminin isoforms containing the alpha-2, -4 and -5 subunits (laminin-211, -221, -411, -421, -521) are critically important for the maintenance and development of heart muscle tissue

  • In the adult heart, the main laminin isoforms expressed are laminin-211 and laminin-221 but laminin-521 has also shown to be expressed

  • Laminin-211 can effectively be used for cardiac differentiation of human iPSC and also for culture of adult human cardiomyocytes

  • Laminin-521 is also an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes, where laminin-521 supports the fastest human iPSC growth rate, generates the highest number of cardiomyocytes and sustain long-term adhesion (>15 d) during cardiac differentiation

  • Laminin-521 support increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube

  • Laminin-521 support more consistent and reliable differentiation over long-term culture and is the only substrate facilitating high-level fusion following long-term culture

  • Defined and xeno-free cell culture matrix for clinical compliance

  • No lot-to-lot variability for standardized experiments with less variation

  • Scientifically validated in high-impact journals

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG human stem cell matrix makes pluripotent stem cell culture easy in a defined, animal component-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Laminin 521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN (LN521) human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.