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""Laminin-521 shifts away from the paradigm that muscle cells lose their ability to differentiate after culturing. Muscle differentiation on laminin-521 is nothing short of spectacular, yielding increased numbers of nuclei per myotube and improved myotube organization. Surprisingly, muscle cells expanded on laminin-521 maintain their ability to differentiate into myotubes after long-term passaging, better resembling differentiation of freshly isolated cells."
Dr. Christopher Penton, Icagen, Inc., USA
LAMININ KEY ADVANTAGES
Laminin-521 is one of the main isoforms surrounding myogenic progenitors
Culture on laminin-521 gives larger myotubes and higher amounts of nuclei per myotube
Increased differentiation potential without altering the traditional Pax7/MyoD paradigm
More consistent and reliable differentiation over long-term culture
Translatability across several mouse backgrounds, human cells and disease states
Laminin-211 is the most abundant laminin isoform in the basement membrane of adult skeletal muscle
Defined and xeno-free matrix, suitable for cell therapy development and clinical trials
Laminin-521 is a superior substrate for both short-term and long-term myogenic cell culture
During myogenesis, laminin-521 and laminin-511 are the main isoforms surrounding myogenic progenitors (Gawlik & Durbeej, 2011). Indeed, recent data presented by researchers at Icagen Inc. demonstrate that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton et al., 2016). Laminin-521 support increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube. Moreover, Penton et al. show that laminin-521 support more consistent and reliable differentiation over long-term culture and is the only substrate facilitating high-level fusion following long-term culture. Laminin-521 support increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells and disease states. Counterintuitively, culture of satellite cell-derived myoblasts on laminin-211, the native laminin isoform in resting skeletal muscle, result in low proliferation and poor differentiation.
Laminin-211 is the most abundant laminin isoform in adult skeletal muscle
Laminin-211 is the most abundant laminin isoform in the basement membrane of adult skeletal muscle with integrin α7β1 as its major receptor. Laminin is crucial for normal muscle function, evident from naturally occurring mutations in laminin genes. Mutations in the gene encoding laminin a2 chain are the most common cause of congenital muscular dystrophy type 1A. For a review on laminin-211 in skeletal muscle function, see Holmberg & Durbeej, 2013.
Laminin-421 and laminin-111 are also expressed in adult skeletal muscle in small amounts, preferably at junctional regions.
Laminin β-2 immunohistochemical staining of
human skeletal muscle show strong membranous
immunoreactivity in myocytes. Anti-LAMB2 antibody
AMAb91096 (Atlas Antibodies).
Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion. Penton et al. Skeletal Muscle, 2016Laminin-211 in skeletal muscle function. Holmberg & Durbeej, Cell Adh Migr., 2013
Skeletal muscle laminin and MDC1A: pathogenesis and treatment strategies. Gawlik & Durbeej. Skelet Muscle, 2011
Distribution of ten laminin chains in dystrophic and regenerating muscles. Patton et al. Neuromuscul Disord., 1999