PRODUCT RECOMMENDATION

LAMININ-521 and LAMININ-211 


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PROTOCOL

Long-term expansion and differentiation of myogenic progenitors on laminin

  

CUSTOMER TESTIMONIALS

"Laminin-521 shifts away from the paradigm that muscle cells lose their ability to differentiate after culturing. Muscle differentiation on laminin-521 is nothing short of spectacular, yielding increased numbers of nuclei per myotube and improved myotube organization. Surprisingly, muscle cells expanded on laminin-521 maintain their ability to differentiate into myotubes after long-term passaging, better resembling differentiation of freshly isolated cells."

                                           Dr. Christopher Penton, Icagen, Inc., USA

         
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ARTICLES

LAMININ KEY ADVANTAGES

  • Laminin-211 is the most abundant laminin isoform in the basement membrane of adult muscle

  • Laminin-211 has been shown to contribute to maintaining muscle stem cells (MuSCs) in a potent, quiescent state in vitro and enhanced potency in vivo

  • Culture of MDC1A myotubes on recombinant laminin-211 restores the mitochondrial function
  • Laminin-521 is one of the main isoforms surrounding myogenic progenitors 

  • Culture on laminin-521 gives larger myotubes and higher amounts of nuclei per myotube

  • Increased differentiation potential on laminin-521 without altering the traditional Pax7/MyoD paradigm

  • More consistent and reliable differentiation on laminin-521, even after long-term culture

  • Translatability across several mouse backgrounds, human cells and disease states

  • Defined and xeno-free matrix, suitable for cell therapy development and clinical trials

 


 Get faster proliferation and better differentiation of skeletal muscle cell culture on human recombinant laminin-521Laminin-521 is a superior substrate for both short-term and long-term myogenic cell expansion and differentiation

During myogenesis, laminin-521 and laminin-511 are the main isoforms surrounding myogenic progenitors (Gawlik & Durbeej, 2011). Indeed, recent data presented by researchers at Icagen Inc. demonstrate that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). Laminin-521 support increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube. Moreover, Penton et al. show that laminin-521 support more consistent and reliable differentiation over long-term culture and is the only substrate facilitating high-level fusion following long-term culture. Laminin-521 support increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells and disease states. 

Researchers in Stanford that developed artificial muscle fibers (AMFs) that mimic the native myofiber of the MuSC niche, show that laminin-211 maintain muscle stem cells (MuSCs) in a potent, quiescent state in vitro. AMFs coated with recombinant laminin-211 showed prolonged quiescence in vitro and enhanced potency in vivo (Quarta, 2016).

 
Laminin-211 is an important part of the adult skeletal muscle microenvironment

Laminin-211 and laminin-221 are the most abundant laminin isoform in the basement membrane of adult skeletal muscle with integrin α7β1 as main receptor (Holmberg & Durbeej, 2013). Laminin-211 is located within the basement membranes that surrounds the sarcolemma, whereas laminin-221 predominates in the neuromuscular and myotendionous junctions. Laminin-421 and laminin-111 are also expressed in adult skeletal Laminin expression in human muscle tissuemuscle in small amounts, preferably at junctional regions (Rogers & Nishimune, 2016). Laminin is crucial for normal muscle function, evident from naturally occurring mutations in laminin genes. Mutations in the gene encoding laminin a2 chain are the most common cause of congenital muscular dystrophy type 1A (Gawlik & Durbeej, 2011). In a publication by Fontes-Oliveira et al. (2017), the authors show that the absence of laminin α2 chain leads to downregulated PGC1α expression, which impairs mitochondrial biogenesis, causing a reduction of mitochondrial content that finally leads to a bioenergetic inefficiency in myoblasts and myotubes from MDC1A patients. By culturing MDC1A myotubes on recombinant laminin-211, the mitochondrial function is restored. Basal respiration, maximum respiration and ATP production as well as basal mitochondrial respiration and maximal mitochondrial respiration capacity are normalized to control levels in the presence of laminin-211.

Due to their vital role for muscle tissue structure, maintenance and function, there is much focus on laminin as a important tool in protocols aiming to improve cell therapy for muscular deceases. Moreover, intramuscular injection of laminin has shown to improve muscle regeneration and efficiency of myoblast transplantation (Riederer, 2015). 

Laminin β-2 immunohistochemical staining of
human skeletal muscle show strong membranous
immunoreactivity in myocytes. Anti-LAMB2 antibody
AMAb91096 (Atlas Antibodies).

RECOMMENDED PUBLICATIONS

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Intestinal cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Animal stem cells

  • Endothelial cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Laminin-521

Laminin-521 human stem cell matrix makes pluripotent stem cell culture easy in a defined, xeno-free and biologically relevant cell culture system.

  • x
  • Neural cells

  • Hepatic cells

  • Lung cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Skeletal muscle cells

  • Kidney cells

  • Cancer cells

  • Pancreatic cells

  • Hematopoietic stem and progenitor cells

  • Animal stem cells

  • Endothelial cells

  • Epithelial cells

Laminin-111

Laminin-111 is important during early epithelial development and differentiation of the epiblast, and is commonly used as a general attachment protein in cell culture.

  • x
  • Neural cells

  • Eye cells

  • Endothelial cells

  • Animal stem cells

  • Pancreatic cells

  • Epithelial cells

  • Cardiac cells

  • Cancer cells

  • Hepatic cells

  • Intestinal cells

  • Kidney cells

  • Lung cells

  • Skeletal muscle cells

  • Expansion of human PSC

  • Clonal cell culture applications

  • Hematopoietic stem and progenitor cells

  • Normal and cancerous mammary cells

LAMscreen

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.