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LAMININ-521 and LAMININ-211 


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PROTOCOL

Long-term expansion and differentiation of myogenic progenitors on laminin


CUSTOMER TESTIMONIALS

"Laminin-521 shifts away from the paradigm that muscle cells lose their ability to differentiate after culturing. Muscle differentiation on laminin-521 is nothing short of spectacular, yielding increased numbers of nuclei per myotube and improved myotube organization. Surprisingly, muscle cells expanded on laminin-521 maintain their ability to differentiate into myotubes after long-term passaging, better resembling differentiation of freshly isolated cells."

                                           Dr. Christopher Penton, Icagen, Inc., USA

         
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ARTICLES

HOW TO CULTURE MUSCLE CELLS ON BIOLAMININ SUBSTRATES

 Get faster proliferation and better differentiation of skeletal muscle cell culture on human recombinant laminin-521Biolaminin 521 is a superior substrate for both short-term and long-term myogenic cell expansion and differentiation

During myogenesis, laminin 521 and laminin 511 are the main isoforms surrounding myogenic progenitors (Gawlik & Durbeej, 2011). Indeed, recent data presented by researchers at Icagen Inc. demonstrate that the Biolaminin 521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). Biolaminin 521 support increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube. Moreover, Penton et al. show that Biolaminin 521 support more consistent and reliable differentiation over long-term culture and is the only substrate facilitating high-level fusion following long-term culture. Biolaminin 521 support increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells and disease states. 

Researchers in Stanford that developed artificial muscle fibers (AMFs) that mimic the native myofiber of the MuSC niche, show that Biolaminin 211 maintain muscle stem cells (MuSCs) in a potent, quiescent state in vitro. AMFs coated with recombinant Biolaminin 211 showed prolonged quiescence in vitro and enhanced potency in vivo (Quarta, 2016). 

Laminin 211 is an important part of the adult skeletal muscle microenvironment

Laminin 211 and laminin 221 are the most abundant laminin isoform in the basement membrane of adult skeletal muscle with integrin α7β1 as main receptor (Holmberg & Durbeej, 2013). Laminin 211 is located within the basement membrane that surrounds the sarcolemma, whereas laminin 221 predominates in the neuromuscular and myotendionous junctions. Laminin 421 and laminin 111 are also expressed in adult skeletal muscle in small amounts, preferably at junctional regions (Rogers & Nishimune, 2016). 

Laminin is crucial for normal muscle function, evident from naturally occurring mutations in laminin genes. Mutations in the gene encoding laminin a2 chain are the most common cause of congenital muscular dystrophy type 1A (Gawlik & Durbeej, 2011). In a publication by Fontes-Oliveira et al. (2017), the authors show that the absence of laminin α2 chain leads to downregulated PGC1α expression, which impairs mitochondrial biogenesis, causing a reduction of mitochondrial content that finally leads to a bioenergetic inefficiency in myoblasts and myotubes from MDC1A patients. By culturing MDC1A myotubes on recombinant Biolaminin 211, the mitochondrial function is restored. Laminin expression in human muscle tissue Basal respiration, maximum respiration and ATP production as well as basal mitochondrial respiration and maximal mitochondrial respiration capacity are normalized to control levels in the presence of laminin 211.

Due to their vital role for muscle tissue structure, maintenance and function, laminin serve as an important tool in protocols aiming to improve cell therapy for muscular deceases. Moreover, intramuscular injection of laminin has shown to improve muscle regeneration and efficiency of myoblast transplantation (Riederer, 2015). 


Laminin β-2 immunohistochemical staining of
human skeletal muscle show strong membranous
immunoreactivity in myocytes. Anti-LAMB2 antibody
AMAb91096 (Atlas Antibodies).

BIOLAMININ KEY ADVANTAGES

  • Laminin 211 is the most abundant laminin isoform in the basement membrane of adult muscle

  • Laminin 211 has been shown to contribute to maintaining muscle stem cells (MuSCs) in a potent, quiescent state in vitro and enhanced potency in vivo

  • Culture of MDC1A myotubes on recombinant Biolaminin 211 restores the mitochondrial function
  • Laminin 521 is one of the main isoforms surrounding myogenic progenitors 

  • Culture on Biolaminin 521 gives larger myotubes and higher amounts of nuclei per myotube

  • Increased differentiation potential on Biolaminin 521 without altering the traditional Pax7/MyoD paradigm

  • More consistent and reliable differentiation on Biolaminin 521, even after long-term culture

  • Translatability across several mouse backgrounds, human cells and disease states

  • Defined and animal component-free matrix, suitable for cell therapy development and clinical trials

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 CTG

Biolaminin 521 CTG cell therapy grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Mesenchymal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 MX

Biolaminin 521 MX research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.

  • x
  • Expansion of human PSC

  • Mesenchymal stem cells

  • Clonal cell culture applications

  • Eye cells

  • Cardiac cells

  • Neural cells

  • Skeletal muscle cells

  • Kidney cells

  • Hepatic cells

  • Cancer cells

  • Lung cells

  • Animal stem cells

  • Endothelial cells

  • Pancreatic cells

  • Intestinal cells

  • Normal and cancerous mammary cells

  • Epithelial cells

Biolaminin 521 LN

Biolaminin 521 LN research grade cell culture matrix makes pluripotent stem cell culture easy. A defined, animal component-free and biologically relevant cell culture system for better cell models.