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STEM CELL CULTURE MATRICES

 - A practical guide 

 

Selecting the right cell culture substrate is often a painstaking and time-consuming task, because of the wide variety of products on the market, the lack of transparency when it comes to various components, and a large number of variables to consider for each application.

This guide was created to help demystify the choices and simplify the selection process. 

Stem cell culture matric paperTo develop a differentiation protocol that generates the target cell type at a sufficient quantity and purity and with high phenotypic maturity is challenging. Following this, the advancement from pre-clinical studies to a Phase 1 clinical trial requires a well-controlled production process and a safe and efficacious cell product.

Lessons from developmental biology have resulted in many protocols that successfully recapitulate the temporal developmental processes via well designed medium formulations. Now, slowly, the important role of the extra-cellular matrix in shaping the cell culture microenvironment is also getting its rightful recognition. Adhesion is not only about making cells stick, but also about the interaction between the culture substrate and cells which significantly influence cell behavior.

 

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