Passaging and culture of human PSC on Biolaminin 521
This video describes how to culture human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) on human recombinant BioLaminin 521 substrates. The protocol can easily be made totally defined and animal component-free with your choice of culture medium since the BioLaminin 521 matrix works well in combination with most commercial media brands. Both enzyme and EDTA based dissociation can be used. Human pluripotent stem cells can routinely be cultured as single cells on BioLaminin 521 without need for ROCK inhibitor (ROCKi).
Watch as cells grow -
Culture ES and iPS cells on laminin
Stem cell culturing of human ES and iPS cells on recombinant laminin matrices is easy and efficient. Human ES and iPS cells grow as monolayers in a completely defined and animal component-free cell culture environment that enables self-renewal and pluripotency without the use of ROCK inhibitors. Here, we show hES cells plated as single cells on top of recombinant laminin-521, growing from day 0 to day 5 when they are ready to be split again. During the first 1-2 days, a picture was taken every 15 minutes. Days 3-5 a picture was taken every hour.