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Technical guides

Benefits of the Biolaminin 521 cell culture system 

This video describes the key benefits of the Biolaminin 521 culture system for defined, animal component-free and feeder-free maintenance and lineage-specific differentiation of human pluripotent stem cells.

                                                                                                  2018-05-08

Coating cultureware with Biolaminin substrates

This video describes how to coat cultureware with Biolaminin, human recombinant laminin substrates, for culture of human pluripotent stem cells, progenitors and various specialised cell types.

                                                                                                  2018-05-08

 

Passaging and culture of human PSC on Biolaminin 521

This video describes how to culture human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) on human recombinant BioLaminin 521 substrates. The protocol can easily be made totally defined and animal component-free with your choice of culture medium since the BioLaminin 521 matrix works well in combination with most commercial media brands. Both enzyme and EDTA based dissociation can be used. Human pluripotent stem cells can routinely be cultured as single cells on BioLaminin 521 without need for ROCK inhibitor (ROCKi).

                                                                                                  2018-05-08

hPSC transition to Biolaminin 521

This video describes how to successfully transition human pluripotent stem cells to Biolaminin 521 from feeder cells or other feeder-free substrates.

                                                                                                  2018-05-08

Watch as cells grow -
Culture ES and iPS cells on laminin

Stem cell culturing of human ES and iPS cells on recombinant laminin matrices is easy and efficient. Human ES and iPS cells grow as monolayers in a completely defined and animal component-free cell culture environment that enables self-renewal and pluripotency without the use of ROCK inhibitors. Here, we show hES cells plated as single cells on top of recombinant laminin-521, growing from day 0 to day 5 when they are ready to be split again. During the first 1-2 days, a picture was taken every 15 minutes. Days 3-5 a picture was taken every hour.

                                                                                                  2014-04-16